TY - JOUR AB - Studies have shown that topiramate (TPM)-induced weight loss can be dependent on the central nervous system (CNS). However, the direct action of TPM on adipose tissue has not been tested previously. Thus, the present study aimed to examine whether TPM modulates lipolysis in 3T3‑L1. The 3T3‑L1 cells were incubated in 50 µM TPM for 30 min. The β‑adrenergic stimulator, isoproterenol, was used as a positive control. The release of lactate dehydrogenase, non‑esterified fatty acid, glycerol and incorporation of 14C‑palmitate to lipid were analyzed. The phosphorylation of protein kinase A (PKA), hormone‑sensitive lipase (HSL), adipocyte triglyceride lipase (ATGL) and perilipin A, as well as the protein levels of comparative genetic identification 58 (CGI‑58) were assessed. The levels of glycerol and non‑esterified fatty acid increased markedly when the cells were treated with TPM. The TPM effects were similar to the isoproterenol positive control. Additionally, TPM reduced lipogenesis. These results were observed without any change in cell viability. Finally, the phosphorylation of PKA, HSL, ATGL and perilipin A, as well as the protein levels of CGI‑58 were increased compared to the control cells. These results were similar to those observed in the cells treated with isoproterenol. The present results show that TPM increased the phosphorylation of pivotal lipolytic enzymes, which induced lipolysis in 3T3‑L1 adipocytes, suggesting that this drug may act directly in the adipose tissue independent from its effect on the CNS. AD - Laboratory of Exercise Biochemistry and Physiology, Health Sciences Unit, University of Extremo Sul Catarinense, Criciúma, SC 88806‑000, Brazil Immunometabolism Research Group, Institute of Biomedical Sciences, University of Sao Paulo, São Paulo, SP 05508‑900, Brazil School of Physical Education and Sport of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP 14040‑907, Brazil School of Applied Sciences, University of Campinas (UNICAMP), Limeira, SP 13484‑350, Brazil Laboratory of Human Movement, São Judas Tadeu University, São Paulo, SP 03166‑000, Brazil Immunometabolism Research Group, Department of Physical Education, University Estadual Paulista (UNESP), Presidente Prudente, SP 19060‑900, Brazil AU - Martins,Gabriela,Poltronieri Campagnaro AU - Souza,Camila,Oliveira AU - Marques,Scherolin,de Oliveira AU - Luciano,Thais,Fernandes AU - da Silva Pieri,Bruno,Luiz AU - Rosa,José,César AU - da Silva,Adelino,Sanchez Ramos AU - Pauli,José,Rodrigo AU - Cintra,Dennys,Esper AU - Ropelle,Eduardo,Rochete AU - Rodrigues,Bruno AU - de Lira,Fabio,Santos AU - de Souza,Claudio,Teodoro DA - 2015/11/01 DO - 10.3892/br.2015.514 EP - 830 IS - 6 JO - Biomed Rep KW - obesity topiramate 3T3-L1 adipocytes lipolysis weight loss PY - 2015 SN - 2049-9434 2049-9442 SP - 827 ST - Topiramate effects lipolysis in 3T3-L1 adipocytes T2 - Biomedical Reports TI - Topiramate effects lipolysis in 3T3-L1 adipocytes UR - https://doi.org/10.3892/br.2015.514 VL - 3 ER -