TY - JOUR AB - The present study hypothesized that the therapeutic use of ascorbic acid (AsA) in combination with radiation may reduce therapy-related side effects and increase the antitumor effects. The aim of the study was to examine the association between the scavenged activity of AsA and the biological anticancer effect of hydroxyl (OH) radicals generated by X-ray irradiation. Cell survival, DNA fragmentation of human leukemia HL60 cells and the amount of OH radicals were investigated following X-ray irradiation and AsA treatment. The number of living cells decreased, and DNA fragmentation increased at AsA concentrations >1 mM. Electron spin resonance spectra revealed that X-ray irradiation generated OH radicals, which were scavenged by AsA at concentrations >75 µM. The AsA concentration inside the cell was 75 µM when cells underwent extracellular treatment with 5 mM AsA, which significantly induced HL60 cell death even without irradiation. No increase in the number of viable HL60 cells was observed following AsA treatment with irradiation when compared to irradiation alone. In conclusion, the disappearance of the radiation anticancer effects with AsA treatment in combination with radiotherapy for cancer treatment is not a cause for concern. AD - Department of Radiation Science, Graduate School of Health Sciences, Hirosaki University, Hirosaki, Aomori 036-8564, Japan AU - Hosokawa,Yoichiro AU - Saga,Ryo AU - Monzen,Satoru AU - Terashima,Shingo AU - Tsuruga,Eichi DA - 2017/01/01 DO - 10.3892/br.2016.819 EP - 107 IS - 1 JO - Biomed Rep KW - X-ray irradiation ascorbic acid hydroxyl radical cell death radical scavenger PY - 2017 SN - 2049-9434 2049-9442 SP - 103 ST - Ascorbic acid does not reduce the anticancer effect of radiotherapy T2 - Biomedical Reports TI - Ascorbic acid does not reduce the anticancer effect of radiotherapy UR - https://doi.org/10.3892/br.2016.819 VL - 6 ER -