TY - JOUR AB - Members of the Ras superfamily of small G-proteins serve as molecular switches of intracellular signaling pathways. Rac2 is a Rho subfamily GTPase switch that is specifically expressed in hematopoietic cells and regulates AKT activation in cell signaling. Ras activating protein-like 3 (RASAL3) is the recently identified Ras GTPase activating protein (GAP) that is also specifically expressed in hematopoietic cells and stimulates p21ras GTPase activity. The restricted expression of both Rac2 and RASAL3 suggests that they may serve critical roles in hematopoietic cell signaling. Here in the present study demonstrates that the catalytic domain of RASAL3 may also be able to interact with Rac2 and stimulate its GTPase activity in vitro. By contrast, p50 rhoGAP molecules did not markedly affect Rac2 GTPase activity, but did accelerate the activity of other Rho GTPases, including Rac1, RhoA and Cdc42. Collectively, the present results indicate, seemingly for the first time, that GAP activity for Rac2 is regulated by the RasGAP family protein, RASAL3. AD - Department of Life Science, College of Science and Technology, Daejin University, Pocheon-Si, Gyeonggi-Do 11159, South Korea Department of Immunology and Physiology, School of Medicine, Keimyung University, Daegu 42601, South Korea Department of Biological Sciences, Division of Life Sciences, Ulsan National Institute of Science and Technology, Ulsan 44919, South Korea AU - Shin,Yoonjae AU - Kim,Yong ,Woo AU - Kim,Hyemin AU - Shin,Nakyoung AU - Kim,Tae ,Sung AU - Kwon,Taeg ,Kyu AU - Choi,Jang ,Hyun AU - Chang,Jong-Soo DA - 2018/09/01 DO - 10.3892/br.2018.1119 EP - 246 IS - 3 JO - Biomed Rep KW - RASAL3 p50 rhoGAP Rac2 GTP hydrolysis GAP PY - 2018 SN - 2049-9434 2049-9442 SP - 241 ST - RASAL3 preferentially stimulates GTP hydrolysis of the Rho family small GTPase Rac2 T2 - Biomedical Reports TI - RASAL3 preferentially stimulates GTP hydrolysis of the Rho family small GTPase Rac2 UR - https://doi.org/10.3892/br.2018.1119 VL - 9 ER -