TY - JOUR AB - Toxoplasma gondii (T. gondii) is prevalent intracellular parasite and a cause of worldwide infection in the human population. An inhibitory effect of this parasite on cancer growth has been demonstrated in cell culture and animal models. To determine whether the anticancer activities of T. gondii are associated with host immune response, in the current study the reactivity of anti‑T. gondii antiserum with the surface of cancer cell lines was investigated. Anti‑T. gondii antibodies were raised in rabbit and the reaction of this antiserum in comparison with other anti‑parasite antisera (anti‑T. vaginalis, anti‑hydatid cyst fluid, anti‑protoscolices antigens) with mouse melanoma or breast cancer cells lines was investigated using flow cytometry. Anti‑T. gondii antiserum reacted markedly with the surface of mouse melanoma and breast cancer cells, and less so with the normal mouse spleen lymphocytes. Meanwhile, the other anti‑parasite antisera did not react strongly with the surface of cancer cells compared with normal mouse spleen lymphocytes. In summary, it has been demonstrated herein that anti‑T. gondii antiserum may selectively react with the surface of mouse cancer cells but not with normal mouse spleen lymphocytes. Therefore, further study on anti‑Toxoplasma antibodies may be useful for directing the application of selective drug delivery in cancer treatment. AD - Department of Medical Parasitology and Mycology, Isfahan University of Medical Sciences, Isfahan 8179498861, Iran Environment Research Center, Research Institute for Primordial Prevention of Non-Communicable Disease, Isfahan University of Medical Sciences, Isfahan 8179498861, Iran Department of Immunology, Isfahan University of Medical Sciences, Isfahan 8179498861, Iran AU - Mohamadi,Fereshteh AU - Shakibapour,Mahshid AU - Sharafi,Seyedeh,Maryam AU - Andalib,Ali,Reza AU - Tolouei,Sepideh AU - Yousofi Darani,Hossein DA - 2019/03/01 DO - 10.3892/br.2019.1186 EP - 188 IS - 3 JO - Biomed Rep KW - antibodies breast cancer cell surface melanoma Toxoplasma gondii PY - 2019 SN - 2049-9434 2049-9442 SP - 183 ST - Anti-Toxoplasma gondii antibodies attach to mouse cancer cell lines but not normal mouse lymphocytes T2 - Biomedical Reports TI - Anti-Toxoplasma gondii antibodies attach to mouse cancer cell lines but not normal mouse lymphocytes UR - https://doi.org/10.3892/br.2019.1186 VL - 10 ER -