TY - JOUR AB - Histamine is involved in the regulation of collagen metabolism during healing following a myocardial infarction; however, its effects on the intact heart tissue is unknown. The aim of the present study was to determine whether histamine may influence collagen content in cells isolated from intact heart, and to identify the histamine receptor involved in the regulation of collagen deposition. Cells were isolated from intact rat hearts and subjected to identification by flow cytometry. The effects of histamine and its receptor agonists and antagonists were investigated. The heart cells were found to be actin, desmin and vimentin positive. Histamine (used at a concentrations of 1x10‑10‑1x10‑5 M) increased collagen content within the culture and increased the expression of α1 chain of the procollagen type III gene. The H2, H3 and H4 receptor inhibitors ranitidine, ciproxifan and JNJ 7777120 blocked the effect of histamine on collagen content. All tested histamine receptor agonists, viz. 2‑pyridylethylamine dihydrochloride (H1 receptor agonist), amthamine dihydrobromide (H2 receptor agonist), imetit (H3 receptor agonist) and 4‑methylhistamine hydrochloride (H4 receptor agonist), elevated collagen content within the heart myofibroblast cultures. The cells isolated from the intact heart were identified as myofibroblasts. Thus, the results of the present study showed that histamine augmented collagen content in the heart myofibroblast culture by activation of three histamine receptors (H2, H3 and H4). The effect of the amine was also dependent on the activation of collagen type III gene expression. AD - Laboratory of Connective Tissue Metabolism, Department of Pathophysiology, Medical University of Łódź, 90‑752 Łódź, Poland Central Scientific Laboratory, Medical University of Łódź, 92‑215 Łódź, Poland Department of Pathophysiology and Experimental Neuroendocrinology, Medical University of Łódź, 90‑752 Łódź, Poland AU - Piera,Lucyna AU - Szymański,Jacek AU - Juszczak,Marlena AU - Drobnik,Jacek DA - 2021/08/01 DO - 10.3892/br.2021.1447 IS - 2 JO - Biomed Rep KW - collagen cell culture heart histamine histamine receptor myofibroblast PY - 2021 SN - 2049-9434 2049-9442 SP - 71 ST - Histamine is involved in the regulation of collagen content in cultured heart myofibroblasts via H2, H3 and H4 histamine receptors T2 - Biomedical Reports TI - Histamine is involved in the regulation of collagen content in cultured heart myofibroblasts via H2, H3 and H4 histamine receptors UR - https://doi.org/10.3892/br.2021.1447 VL - 15 ER -