TY - JOUR
AB - The demand for L‑asparaginase is predicted to increase several fold in the future due to its potential clinical applications in the treatment of lymphoid system malignancies and leukemia. Thus identifying suitable sources of production should be considered high priority. Fungi are valuable organisms as they are able to convert what would be considered ‘useless’ materials into materials that have potential value. The present study provides a proof of concept of production of a new hyperactive L‑asparaginase producer (Rhizopus oryzae AM16), which was successfully isolated and sequentially optimized using a semi solid‑state fermentation method with a simple and cheap medium produced from wheat bran (WB). The fungus was able to produce an appreciable amount of the enzyme (2,875.9 U) after 8 days of incubation under 85.7% moisture, in the presence of magnesium nitrate (5.0 mg N/mg nitrogen per gram of dry WB) at pH 5.8. Testing the anticancer activity confirmed the ability of the resultant enzyme to inhibit the growth of various types of cancer cells (HepG2, MCF‑7, HCT and A549). The IC50 values of the dialyzed enzyme were lower than that of the crude product. Thus, this newly identified and purified L‑asparaginase may be a promising anticancer drug.
AD - Department of Biology, Faculty of Science, Princess Nourah Bint Abdulrahman University, Riyadh 11671, Saudi Arabia
The Regional Center of Mycology and Biotechnology, Al‑Azhar University, Cairo 11765, Egypt
Botany and Microbiology Department, Faculty of Science, Damanhour University, Damanhour 22511, Egypt
Department of Anatomy and Embryology, Faculty of Medicine, Mansoura University, Mansoura 35511, Egypt
Zoology Department, Faculty of Science, Beni‑Suef University, Beni‑Suef 62511, Egypt
Microbial Activity Unit, Department of Microbiology, Soils, Water and Environment Research Institute, Agricultural Research Center, Giza 12619, Egypt
AU - Othman,Sarah,I.
AU - Mekawey,Amal,A.I.
AU - El‑Metwally,Mohammad,M.
AU - Gabr,Sami,A.
AU - Alwaele,Maha,Abdulla
AU - Al Fassam,Haifa
AU - Abo‑Eleneen,Rasha
AU - Allam,Ahmed,A.
AU - Saber,Wesameldin,I.A.
DA - 2022/03/01
DO - 10.3892/br.2022.1498
IS - 3
JO - Biomed Rep
KW - fermentation
fungi
tumor
leukemia
cancer cell
PY - 2022
SN - 2049-9434
2049-9442
SP - 15
ST - Rhizopus oryzae AM16; a new hyperactive L‑asparaginase producer: Semi solid‑state production and anticancer activity of the partially purified protein
T2 - Biomedical Reports
TI - Rhizopus oryzae AM16; a new hyperactive L‑asparaginase producer: Semi solid‑state production and anticancer activity of the partially purified protein
UR - https://doi.org/10.3892/br.2022.1498
VL - 16
ER -