TY - JOUR AB - Juvenile nasopharingeal angiofibroma (JNA) is a histologically benign locally aggressive tumor characterized by irregular vessels embedded in a fibrous stroma. Excessive vascularity results in bleeding complications, and the inhibition of angiogenesis is a promising strategy for managing extensive JNA tumors. To better characterize the endothelial components of JNA, we aimed to evaluate markers of vascular differentiation and proliferation, such as friend leukemia integration-1 (FLI-1) and endoglin, lymphatic markers, including podoplanin and vascular endothelial growth factor receptor 3 (VEGFR3) and its cognate ligand VEGFC, GLUT-1, a diagnostic marker that discriminates between hemangiomas and vascular malformations, and two markers of tissue remodeling, stromelysin 3 (ST3) and secreted acid protein rich in cysteine (SPARC). Antigens were assessed immunohistochemically in vessels and stromal cells of JNA archival cases (n=22). JNA endothelial cells were positive for endoglin, VEGFC and FLI-1, whereas podoplanin and VEGFR3 were negative in all cases. Both endothelial cells and fibroblasts stained for ST3 and SPARC. GLUT-1 was investigated in JNA cases, in infantile hemangiomas (n=123) and in vascular malformations (n=135) as controls. JNAs and vascular malformations were GLUT-1-negative, while hemangiomas showed positive staining. The presence of markers of endothelial differentiation and proliferation highlighted the hyper-proliferative state of JNA vessels. The absence of podoplanin and VEGFR3 underscores their blood endothelial cell characteristic. The absence of GLUT-1 discriminates JNAs from hemangiomas. ST3 and SPARC up-regulation in endothelial cells and fibroblasts may contribute to a compensatory signaling for controlling angiogenesis. Some of these markers may eventually serve as therapeutic targets. Our results may aid in the understanding of JNA pathophysiology. AD - Instituto Adolfo Lutz, Central, Divisão de Patologia, Laboratório de Imuno-histoquímica, CEP 01246-902, São Paulo, Brasil Hospital A.C. Camargo, CEP 01509-010, São Paulo, Brasil Departamento de Oftalmologia e Otorrinolaringologia, Faculdade de Medicina da Universidade de São Paulo, Cerqueira César CEP 05403-010, São Paulo, Brasil Departamento de Radiologia e Oncologia, Disciplina de Oncologia, Faculdade de Medicina da Universidade de São Paulo (LIM-24), CEP 01246-903, São Paulo, Brasil AU - Nonogaki,Suely AU - Campos,Heloisa ,G.A. AU - Butugan,Ossamu AU - Soares,Fernando ,A. AU - Rotea Mangone,Flávia,Regina AU - Torloni,Humberto AU - Mitzi Brentani,M. DA - 2010/11/01 DO - 10.3892/etm.2010.141 EP - 926 IS - 6 JO - Exp Ther Med KW - juvenile nasopharingeal angiofibroma endoglin friend leukemia integration-1 podoplanin vascular endothelial growth factor receptor 3 GLUT-1 stromelysin 3 secreted acid protein rich in cysteine PY - 2010 SN - 1792-0981 1792-1015 SP - 921 ST - Markers of vascular differentiation, proliferation and tissue remodeling in juvenile nasopharyngeal angiofibromas T2 - Experimental and Therapeutic Medicine TI - Markers of vascular differentiation, proliferation and tissue remodeling in juvenile nasopharyngeal angiofibromas UR - https://doi.org/10.3892/etm.2010.141 VL - 1 ER -