TY - JOUR AB - Paeoniflorin (PF) is a product derived from Paeoniae Radix and is commonly prescribed in traditional Chinese medicine. PF has been reported to exhibit neuroprotective, anti‑ischemic, antioxidant, anti‑inflammatory and anticancer effects. The neuroprotective properties of PF have been demonstrated in animal models of various neuropathologies. The present study investigated the effects of PF on hydrogen peroxide (H2O2)‑induced apoptosis in PC12 cells, to improve the understanding of the mechanisms underlying its neuroprotective properties. The H2O2‑induced apoptosis of PC12 cells resulted in a reduction in the B‑cell lymphoma 2 (Bcl‑2)/Bcl‑2‑associated X protein ratio and the activation of caspase‑3. PF treatment was observed to reverse the apoptotic process and to modulate the expression levels of a number of apoptosis‑associated proteins. Furthermore, PF significantly mitigated the H2O2‑induced reduction in cell viability, in addition to scavenging reactive oxygen species and preventing the release of lactate dehydrogenase from the PC12 cells. In addition, the apoptosis‑associated activation of nuclear factor (NF)‑κB was inhibited in the PF‑treated cells, and the expression levels of tumor necrosis factor α and interleukin (IL)‑1β were reduced. In conclusion, the present study demonstrated that PF was able to reduce H2O2‑induced toxicity by blocking the activation of the neuroinflammatory factor NF‑κB. These results suggest that PF may be a valuable neuroprotective agent for the treatment of neurological disease and injury. AD - Medical College of Nankai University, Tianjin 300071, P.R. China Department of Ophthalmology, General Hospital of PLA, Beijing 100853, P.R. China AU - Li,Peng AU - Li,Zhaohui DA - 2015/05/01 DO - 10.3892/etm.2015.2360 EP - 1772 IS - 5 JO - Exp Ther Med KW - paeoniflorin neuroprotective effects PC12 cells inflammatory response PY - 2015 SN - 1792-0981 1792-1015 SP - 1768 ST - Neuroprotective effect of paeoniflorin on H2O2-induced apoptosis in PC12 cells by modulation of reactive oxygen species and the inflammatory response T2 - Experimental and Therapeutic Medicine TI - Neuroprotective effect of paeoniflorin on H2O2-induced apoptosis in PC12 cells by modulation of reactive oxygen species and the inflammatory response UR - https://doi.org/10.3892/etm.2015.2360 VL - 9 ER -