TY - JOUR AB - Non‑insulin‑dependent diabetes mellitus (NIDDM) is associated with chronic inflammatory activity and disrupted insulin signaling, leading to insulin resistance (IR). The present study investigated the benefits of neurolytic celiac plexus block (NCPB) on IR in a rat NIDDM model. Goto‑Kakizaki rats fed a high‑fat, high‑glucose diet to induce signs of NIDDM were randomly divided into NCPB and control groups; these received daily bilateral 0.5% lidocaine or 0.9% saline injections into the celiac plexus, respectively. Following 14 and 28 daily injections, rats were subject to oral glucose tolerance tests (OGTTs) or sacrificed for the analysis of serum free fatty acids (FFAs), serum inflammatory cytokines and skeletal muscle insulin signaling. Compared with controls, rats in the NCPB group demonstrated significantly (P<0.05) lower baseline, 60‑min and 120‑min OGTT values, lower 120‑min serum insulin, lower IR [higher insulin sensitivity index (ISI1) and lower ISI2) and lower serum FFAs, tumor necrosis factor‑α, interleukin (IL)‑1β and IL‑6. Conversely, NCPB rats exhibited higher basal and insulin‑stimulated skeletal muscle glucose uptake and higher skeletal muscle insulin receptor substrate‑1 (IRS‑1) and glucose transporter type 4 expression. There were no differences between the groups in insulin receptor β (Rβ) or Akt expression; however Rβ‑Y1162/Y1163 and Akt‑S473 phosphorylation levels were higher and IRS‑1‑S307 phosphorylation were lower in NCPB rats than in the controls. These results indicate that NCPB improved insulin signaling and reduced IR, possibly by inhibiting inflammatory cytokine release. AD - Department of Anesthesiology, Chengdu Military General Hospital, Chengdu, Sichuan 610083, P.R. China General Surgery Center, Chengdu Military General Hospital, Chengdu, Sichuan 610083, P.R. China Medical Laboratory Center, Chengdu Military General Hospital, Chengdu, Sichuan 610083, P.R. China Department of Urology, Chengdu Military General Hospital, Chengdu, Sichuan 610083, P.R. China Development and Regeneration Key Laboratory of Sichuan, Chengdu Medical College, Chengdu, Sichuan 610083, P.R. China Department of ICU, Chengdu Military General Hospital, Chengdu, Sichuan 610083, P.R. China AU - Li,Jun AU - Chen,Tao AU - Li,Kun AU - Yan,Hongtao AU - Li,Xiaowei AU - Yang,Yun AU - Zhang,Yulan AU - Su,Bingyin AU - Li,Fuxiang DA - 2016/05/01 DO - 10.3892/etm.2016.3087 EP - 2041 IS - 5 JO - Exp Ther Med KW - neurolytic celiac plexus block Goto‑Kakizaki rats insulin resistance insulin signal transduction pathway PY - 2016 SN - 1792-0981 1792-1015 SP - 2033 ST - Neurolytic celiac plexus block enhances skeletal muscle insulin signaling and attenuates insulin resistance in GK rats T2 - Experimental and Therapeutic Medicine TI - Neurolytic celiac plexus block enhances skeletal muscle insulin signaling and attenuates insulin resistance in GK rats UR - https://doi.org/10.3892/etm.2016.3087 VL - 11 ER -