TY - JOUR AB - Immunogenic cell death (ICD) is characterized by the early surface exposure of calreticulin (CRT). As a specific signaling molecule, CRT on the surface of apoptotic tumor cells mediates the recognition and phagocytosis of tumor cells by antigen presenting cells. To date, only a small quantity of anti-cancer chemicals have been found to induce ICD, therefore it is clinically important to identify novel chemicals that may induce ICD. The purpose of the present study is to explore the function of capsaicin in inducing ICD. In the current study, MTT assays were used to examine the growth inhibiting effects of MG‑63 cells when they were treated with capsaicin or cisplatin. Mitochondrial membrane potential and western blot analysis were used to investigate capsaicin‑ and cisplatin‑induced apoptosis. In addition, the effects of capsaicin and cisplatin were evaluated for their abilities in inducing calreticulin membrane translocation and mediating ICD in human osteosarcoma cells (MG‑63). The results demonstrated that capsaicin and cisplatin can induce the apoptosis of MG‑63 cells. However, only capsaicin induced a rapid translocation of CRT from the intracellular space to the cell surface. Treatment with capsaicin increased phagocytosis of MG-63 cells by dendritic cells (DCs), and these MG‑63‑loaded DCs could efficiently stimulate the secretion of IFN‑γ by lymphocytes. These results identify capsaicin as an anti‑cancer agent capable of inducing ICD in human osteosarcoma cells in vitro. AD - Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China Laboratory of Tumor Microenvironment and Immunotherapy, Medical College of Three Gorges University, Yichang, Hubei 443002, P.R. China AU - Jin,Tao AU - Wu,Hongyan AU - Wang,Yanlin AU - Peng,Hao DA - 2016/08/01 DO - 10.3892/etm.2016.3368 EP - 770 IS - 2 JO - Exp Ther Med KW - capsaicin immunogenic cell death calreticulin osteosarcoma tumor specific immune response dendritic cells phagocytosis assays PY - 2016 SN - 1792-0981 1792-1015 SP - 765 ST - Capsaicin induces immunogenic cell death in human osteosarcoma cells T2 - Experimental and Therapeutic Medicine TI - Capsaicin induces immunogenic cell death in human osteosarcoma cells UR - https://doi.org/10.3892/etm.2016.3368 VL - 12 ER -