TY - JOUR AB - Articular cartilage defects are a major clinical burden worldwide. Current methods to repair bone defects include bone autografts, allografts and external fixation. In recent years, the repair of bone defects by tissue engineering has emerged as a promising approach. The present study aimed to assess a novel method using a biological reactor with platelet‑rich plasma to construct tissue‑engineered bone. Beagle bone marrow mesenchymal stem cells (BMSCs) were isolated and differentiated into osteoblasts and chondroblasts using platelet‑rich plasma and tricalcium phosphate scaffolds cultured in a bioreactor for 3 weeks. The cell scaffold composites were examined by scanning electron microscopy (SEM) and implanted into beagles with articular cartilage defects. The expression of osteogenic markers, alkaline phosphatase and bone γ‑carboxyglutamate protein (BGLAP) were assessed using polymerase chain reaction after 3 months. Articular cartilage specimens were observed histologically. Adhesion and distribution of BMSCs on the β‑tricalcium phosphate (β‑TCP) scaffold were confirmed by SEM. Histological examination revealed that in vivo bone defects were largely repaired 12 weeks following implantation. The expression levels of alkaline phosphatase (ALP) and BGLAP in the experimental groups were significantly elevated compared with the negative controls. BMSCs may be optimum seed cells for tissue engineering in bone repair. Platelet‑rich plasma (PRP) provides a rich source of cytokines to promote BMSC function. The β‑TCP scaffold is advantageous for tissue engineering due to its biocompatibility and 3D structure that promotes cell adhesion, growth and differentiation. The tissue‑engineered bone was constructed in a bioreactor using BMSCs, β‑TCP scaffolds and PRP and displayed appropriate morphology and biological function. The present study provides an efficient method for the generation of tissue‑engineered bone for cartilage repair, compared with previously used methods. AD - Department of Orthopedics, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China Department of Orthopedics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China AU - Li,Huibo AU - Sun,Shui AU - Liu,Haili AU - Chen,Hua AU - Rong,Xin AU - Lou,Jigang AU - Yang,Yunbei AU - Yang,Yi AU - Liu,Hao DA - 2016/08/01 DO - 10.3892/etm.2016.3380 EP - 719 IS - 2 JO - Exp Ther Med KW - bone repair bone tissue engineering bone regeneration bioreactor platelet‑rich plasma PY - 2016 SN - 1792-0981 1792-1015 SP - 711 ST - Use of a biological reactor and platelet-rich plasma for the construction of tissue-engineered bone to repair articular cartilage defects T2 - Experimental and Therapeutic Medicine TI - Use of a biological reactor and platelet-rich plasma for the construction of tissue-engineered bone to repair articular cartilage defects UR - https://doi.org/10.3892/etm.2016.3380 VL - 12 ER -