TY - JOUR AB - MicroRNA-155 (miR-155) is overexpressed in numerous human cancer types and has an oncogenic role. Previous study has revealed that miR‑155 serves an important role in the progression of clear‑cell renal cell carcinoma (ccRCC); however, the underlying mechanism was not completely clarified. The present study aimed to investigate the biological role of miR‑155 in ccRCC and the underlying molecular mechanisms. The expression of miR‑155 in 20 ccRCC and adjacent normal kidney tissues was determined by PCR. After downregulation of miR‑155 expression by miR‑155 inhibitor, cell growth was assessed by MTT and colony formation assays. Apoptosis and cell cycle distribution were analyzed by flow cytometry. Cell invasion and migration was detected by wound healing and Transwell assays. Furthermore, forkhead box O3a (FOXO3a) mRNA and protein expression were detected by PCR and immunoblotting. The expression of FOXO3a in 20 ccRCC tissues was also examined by immunohistochemistry. The expression of miR‑155 was upregulated in ccRCC tissues compared to that in adjacent normal tissues. Inhibition of miR‑155 significantly suppressed the proliferation, colony formation, migration and invasion, and induced G1 arrest and apoptosis of ccRCC cells in vitro. Moreover, inhibition of miR‑155 significantly upregulated FOXO3a expression, and miR‑155 expression was inversely correlated with FOXO3a expression in ccRCC tissues. In conclusion, miR‑155 may have an important role in the genesis of ccRCC through targeting FOXO3a and may be a potential target for ccRCC therapy. AD - Department of Pathology, Binzhou Medical University Hospital, Binzhou, Shandong 256600, P.R. China Department of Urology, Binzhou Medical University Hospital, Binzhou, Shandong 256600, P.R. China AU - Ji,Hong AU - Tian,Dong AU - Zhang,Bing AU - Zhang,Yangyang AU - Yan,Dongliang AU - Wu,Shuhua DA - 2017/05/01 DO - 10.3892/etm.2017.4263 EP - 2292 IS - 5 JO - Exp Ther Med KW - renal cell carcinoma miR‑155 FOXO3a tumorigenesis PY - 2017 SN - 1792-0981 1792-1015 SP - 2286 ST - Overexpression of miR‑155 in clear‑cell renal cell carcinoma and its oncogenic effect through targeting FOXO3a T2 - Experimental and Therapeutic Medicine TI - Overexpression of miR‑155 in clear‑cell renal cell carcinoma and its oncogenic effect through targeting FOXO3a UR - https://doi.org/10.3892/etm.2017.4263 VL - 13 ER -