TY - JOUR AB - Metastasis frequently occurs in advanced ovarian cancer, which not only leads to substantial mortality but also becomes a major challenge to effective treatment. Epithelial‑mesenchymal transition (EMT) is a key mechanism facilitating cancer metastasis. Targeting the EMT process with more efficacious and less toxic agents to prevent metastasis is of significant therapeutic value for ovarian cancer treatment. The anti‑EMT function and mechanism of ginsenoside Rb1, a monomer composition extracted from the traditional Chinese herb Panax ginseng or P. notoginseng, was investigated in the present study. Western blotting demonstrated that treatment with ginsenoside Rb1 antagonized hypoxia‑induced E‑cadherin downregulation and vimentin upregulation in SKOV3 and 3AO human ovarian cancer cells. Wound healing assays and in vitro migration assays indicated that ginsenoside Rb1 weakened hypoxia‑enhanced cell migration ability. Furthermore, it was demonstrated that microRNA (miR)‑25 is upregulated by hypoxia in ovarian cancer cells, which was attenuated by ginsenoside Rb1 treatment. Additionally, forced expression of miR‑25 in ovarian cancer cells was identified to not only trigger EMT, but also block the suppressive effects of ginsenoside Rb1 on hypoxia‑induced EMT by negatively targeting the E‑cadherin transactivator, EP300. In conclusion, ginsenoside Rb1 may reverse hypoxia‑induced EMT by abrogating the suppression of miR‑25 on EP300 and E‑cadherin, which suggests that ginsenoside Rb1 may be a potential therapeutic candidate for the treatment of ovarian cancer. AD - Center for Translational Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China Center for Laboratory Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China AU - Liu,Dan AU - Liu,Ting AU - Teng,Yue AU - Chen,Wei AU - Zhao,Le AU - Li,Xu DA - 2017/10/01 DO - 10.3892/etm.2017.4889 EP - 2902 IS - 4 JO - Exp Ther Med KW - ginsenoside ovarian cancer epithelial‑mesenchymal transition hypoxia microRNA PY - 2017 SN - 1792-0981 1792-1015 SP - 2895 ST - Ginsenoside Rb1 inhibits hypoxia-induced epithelial‑mesenchymal transition in ovarian cancer cells by regulating microRNA-25 T2 - Experimental and Therapeutic Medicine TI - Ginsenoside Rb1 inhibits hypoxia-induced epithelial‑mesenchymal transition in ovarian cancer cells by regulating microRNA-25 UR - https://doi.org/10.3892/etm.2017.4889 VL - 14 ER -