TY - JOUR AB - The objective of the present study was to investigate the role of Toll‑like receptor (TLR)‑9 in B lymphocyte stimulating factor (BLyS)‑induced systemic lupus erythematosus (SLE) in mice. The anti‑double stranded (ds)DNA antibody titer, levels of complement proteins (C3 and C4), interleukin (IL)‑10 and the disease activity [assessed by the erythrocyte sedimentation rate (ESR) and C‑reactive protein (CRP) level] were measured. A total of 21 transgenic female mice (aged 8‑10 weeks and weighing 30‑40 g) expressing the Epstein‑Barr virus membrane antigen, BLLF1, were studied. Mice were randomly divided into the control, the BLyS inhibition and the TLR‑9 inhibition groups, with 7 mice in each group. Mice in the blank control group received intraperitoneal injections of normal saline, mice in the BLyS inhibition group received intraperitoneal injections of anti‑BR3 monoclonal antibody (5,000 ng/day) and mice in the TLR‑9 inhibition group received intraperitoneal injections of anti‑human TLR‑9 antibody (250 ng/day). The treatment regimens continued for 10 days, followed by the collection of peripheral venous blood. The relative levels of TLR‑9 mRNA were measured by reverse transcription‑quantitative polymerase chain reaction. Furthermore, the BLyS protein concentration and IL‑10 levels were measured by ELISA. TLR‑9 mRNA, BLyS, IL‑10, anti‑dsDNA antibody titer, C3, C4, ESR and CRP levels of the blank control group were significantly higher than those of the other two groups (P<0.05). The differences in comparison of these indexes between the BLyS inhibition and TLR‑9 inhibition groups were not statistically significant (P>0.05), with the exception of TLR‑9 mRNA and BLyS. In conclusion, the TLR‑9 signaling pathway may be important for BLyS‑induced SLE, and regulation of the inflammatory immune level. AD - Graduate School of Southern Medical University, Guangzhou, Guangdong 510515, P.R. China Department of Rheumatology and Clinical Immunology, Hainan General Hospital, Haikou, Hainan 570000, P.R. China AU - Liu,Ying AU - Zhan,Feng AU - Zhang,Xiao AU - Lin,Shudian DA - 2018/01/01 DO - 10.3892/etm.2017.5411 EP - 591 IS - 1 JO - Exp Ther Med KW - toll‑like receptor signal B lymphocyte stimulating factor systemic lupus erythematosus signal pathway double stranded DNA interleukin‑10 PY - 2018 SN - 1792-0981 1792-1015 SP - 585 ST - Toll-like receptor-9 is involved in the development of B cell stimulating factor-induced systemic lupus erythematosus T2 - Experimental and Therapeutic Medicine TI - Toll-like receptor-9 is involved in the development of B cell stimulating factor-induced systemic lupus erythematosus UR - https://doi.org/10.3892/etm.2017.5411 VL - 15 ER -