TY - JOUR AB - The aim of the present study was to explore the effects and mechanisms of insulin on mitochondrial oxidative stress in septic acute kidney injury (AKI). Male Sprague Dawley rats were divided randomly into four groups: Control group, sham surgery group, cecal ligation and puncture (CLP) group, and CLP plus insulin group. Blood specimens and kidney tissues were obtained at 12 and 24 h after surgery as separate experiments. Analyses of histology and indicators of renal injury [blood urea nitrogen (BUN) and serum creatinine (CRE) and neutrophil gelatinase‑associated lipocalin (NGAL)], mitochondrial function [adenosine triphosphate (ATP) and mitochondrial membrane potential (MMP)], oxidative stress [inducible nitric oxide synthase (iNOS), reactive oxygen species (ROS) and nitric oxide (NO)], endogenous antioxidant systems [superoxide dismutase (SOD) and glutathione (GSH)] as well as the expression of uncoupling protein (UCP), PINK1 protein (a major mediator of mitophagy), PGC1α protein (a major regulator of mitochondrial biogenesis) were performed. Compared with CLP group, the CLP plus insulin group had milder histological damage, higher levels of ATP and MMP as well as lower levels of BUN, serum CRE and NGAL, intrarenal iNOS, mitochondrial ROS and total NO. Moreover, the CLP plus insulin group demonstrated increased expression of SOD2 and UCP2. In contrast, insulin administration suppressed mitophagy meanwhile did not upregulate total GSH and induce mitochondrial biogenesis following CLP. These findings indicated that the upregulation of SOD2 and UCP2 may be involved in insulin protecting against mitochondrial oxidative stress in septic AKI. AD - Center of Pediatrics, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510280, P.R. China AU - Chen,Guang‑Dao AU - Zhang,Jun‑Liang AU - Chen,Yi‑Ting AU - Zhang,Ju‑Xing AU - Wang,Tao AU - Zeng,Qi‑Yi DA - 2018/04/01 DO - 10.3892/etm.2018.5890 EP - 3975 IS - 4 JO - Exp Ther Med KW - insulin sepsis acute kidney injury mitochondrial dysfunction oxidative stress endogenous antioxidant system uncoupling protein 2 mitophagy mitochondrial biogenesis PY - 2018 SN - 1792-0981 1792-1015 SP - 3967 ST - Insulin alleviates mitochondrial oxidative stress involving upregulation of superoxide dismutase 2 and uncoupling protein 2 in septic acute kidney injury T2 - Experimental and Therapeutic Medicine TI - Insulin alleviates mitochondrial oxidative stress involving upregulation of superoxide dismutase 2 and uncoupling protein 2 in septic acute kidney injury UR - https://doi.org/10.3892/etm.2018.5890 VL - 15 ER -