TY - JOUR AB - Immunofluorescence in situ hybridization (immuno‑FISH) is widely used to co‑detect RNAs and proteins in order to study their spatial distribution in cells. The present study used a modified immuno‑FISH protocol for the detection of long non‑coding RNAs (lncRNAs) and proteins in frozen spinal cord sections. The spinal cords of Sprague‑Dawley rats were harvested, frozen and sectioned (10 µm), and oligonucleotide probes and antibodies were prepared. Following antigen retrieval, dehydration, prehybridization, hybridization, post‑hybridization and immunofluorescence staining, images were captured. Antigen retrieval was performed by autoclaving or proteinase K treatment, and their effects on the hybridization signal were compared. The same sections were successfully stained by immunofluorescence. Satisfactory fluorescent signals of lncRNA and protein were obtained. The results of the present study suggest that the modified protocol of immuno‑FISH for the detection of lncRNAs and proteins in frozen spinal cord sections is effective and time‑efficient, and the required reagents are readily available. AD - Department of Hand Surgery, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China AU - Meng,Chong AU - Zhao,Xin AU - Lao,Jie DA - 2018/06/01 DO - 10.3892/etm.2018.6046 EP - 4628 IS - 6 JO - Exp Ther Med KW - fluorescent in situ hybridization immunofluorescence long non‑coding RNA frozen section antigen retrieval PY - 2018 SN - 1792-0981 1792-1015 SP - 4623 ST - A modified immunofluorescence in situ hybridization method to detect long non‑coding RNAs and proteins in frozen spinal cord sections T2 - Experimental and Therapeutic Medicine TI - A modified immunofluorescence in situ hybridization method to detect long non‑coding RNAs and proteins in frozen spinal cord sections UR - https://doi.org/10.3892/etm.2018.6046 VL - 15 ER -