TY - JOUR AB - Cancer, and particularly colon cancer, is associated with an increasing number of cases resistant to chemotherapy. One approach to overcome this, and to improve the prognosis and outcome of patients, is the use of adjuvant therapy alongside the standard chemotherapy regiment. In the present study, the effect of deuterium‑depleted water (DDW) as a potential modulator of adjuvant therapy on DLD‑1 colorectal cancer models was assessed. A number of functionality assays were performed, including MTT, apoptosis and autophagy, and mitochondrial activity and senescence assays, in addition to assessing the capacity to modify the pattern of released miRNA via microarray technology. No significant effect on cell viability was identified, but an increase in mitochondrial activity and a weak pro‑apoptotic effect were observed in the treated DLD‑1 cells cultured in DDW‑prepared medium compared with those grown in standard conditions (SC). Furthermore, the findings revealed the capacity of DDW medium to promote senescence to a higher degree compared with SC. The exosome‑released miRNA pattern was significantly modified for the cells maintained in DDW compared with those maintained in SC. These findings suggest that DDW may serve as an adjuvant treatment; however, a better understanding of the underlying molecular mechanism of action will be useful for developing novel and efficient therapeutic strategies, in which the transcriptomic pattern serves an important role. AD - Research Center for Functional Genomics, Biomedicine and Translational Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 400337 Cluj‑Napoca, Romania Department of Surgery, Iuliu Hatieganu University of Medicine and Pharmacy, 40015 Cluj‑Napoca, Romania AU - Chira,Sergiu AU - Raduly,Lajos AU - Braicu,Cornelia AU - Jurj,Ancuta AU - Cojocneanu‑Petric,Roxana AU - Pop,Laura AU - Pileczki,Valentina AU - Ionescu,Calin AU - Berindan‑Neagoe,Ioana DA - 2018/08/01 DO - 10.3892/etm.2018.6324 EP - 1249 IS - 2 JO - Exp Ther Med KW - colorectal cancer adjuvant therapy cell death senescence exosomal miRNAs pattern PY - 2018 SN - 1792-0981 1792-1015 SP - 1241 ST - Premature senescence activation in DLD‑1 colorectal cancer cells through adjuvant therapy to induce a miRNA profile modulating cellular death T2 - Experimental and Therapeutic Medicine TI - Premature senescence activation in DLD‑1 colorectal cancer cells through adjuvant therapy to induce a miRNA profile modulating cellular death UR - https://doi.org/10.3892/etm.2018.6324 VL - 16 ER -