TY - JOUR AB - The present study investigated the effect and underlying mechanisms of ginsenoside Rg1 (Rg1) in attenuating subacute liver injury induced by D‑galactose (D‑gal) in mice. Specific Pathogen Free (SPF) male C57BL/6J mice were randomly divided into 3 groups: i) D‑gal‑administration group (D‑gal group), where the mice were intraperitoneally administrated with D‑gal (120 mg/kg/day for 42 days); ii) D‑gal + Rg1 group where the mice were treated with 120 mg/kg/day D‑gal for 42 days and with Rg1 at a dose of 20 mg/kg/day for 35 days. The first dose of Rg1 was administered on the 8th day of treatment with D‑gal; and iii) the normal control group, where the mice were injected with an equal volume of saline for 42 days. The day following the final injections in all groups, peripheral blood was collected and serum was prepared to measure the contents of aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin (TBiL), advanced glycation end products (AGEs) and 8‑hydroxy‑2 deoxyguanosine (8‑OH‑dG). Liver tissue homogenates were prepared to measure the contents of malondialdehyde (MDA) and glutathione (GSH), and the activities of glutathione peroxidase (GSH‑Px) and superoxide dismutase (SOD). Paraffin section were prepared to observe the microscopic structure of the liver. Transmission electron microscopy was used to observe the ultrastructure of hepatocytes. Frozen section were prepared and stained with senescence‑associated β‑galactosidase to detect the relative optical density value of senescence‑associated markers. Compared with the D‑gal group, the contents of AST, ALT, TBiL, AGEs and MDA significantly decreased in the D‑gal + Rg1 group, while the activities of SOD and GSH‑Px markedly increased, and liver injury and degenerative alterations of hepatocytes were reduced. Administration of Rg1 induced a protective effect on D‑gal‑induced liver injury in mice by inhibiting the oxidative stress, reducing DNA damage and decreasing the AGE content. AD - Laboratory of Stem Cells and Tissue Engineering, Department of Histology and Embryology, Chongqing Medical University, Chongqing 400016, P.R. China AU - Xiao,Ming‑He AU - Xia,Jie‑Yu AU - Wang,Zi‑Ling AU - Hu,Wen‑Xu AU - Fan,Yan‑Ling AU - Jia,Dao‑Yong AU - Li,Jing AU - Jing,Peng‑Wei AU - Wang,Lu AU - Wang,Ya‑Ping DA - 2018/11/01 DO - 10.3892/etm.2018.6727 EP - 4106 IS - 5 JO - Exp Ther Med KW - ginsenoside Rg1 D‑galactose liver injury underlying mechanism PY - 2018 SN - 1792-0981 1792-1015 SP - 4100 ST - Ginsenoside Rg1 attenuates liver injury induced by D‑galactose in mice T2 - Experimental and Therapeutic Medicine TI - Ginsenoside Rg1 attenuates liver injury induced by D‑galactose in mice UR - https://doi.org/10.3892/etm.2018.6727 VL - 16 ER -