TY - JOUR AB - Previous experimental models showed that activation of the immune system, particularly T cells, is required for optimal healing following wounds or surgery in the oral cavity. Therefore, studies to explore the interactions between the immune system and the collagen matrix are mandated. The specific aim of the present study was to analyze the interactions between T lymphocytes and a resorbable three-dimensional (3D) collagen matrix routinely used for soft tissue regeneration during periodontal surgery. Peripheral venous blood samples were collected from five patients. Following Ficoll-Paque separation, mononuclear cells were grown on fully resorbable 3D collagen matrices for 5 days. Lymphocytes were analyzed by flow cytometry for different surface markers, including CD4, CD8, CD38 and CD69. Cell viability and late apoptosis/necrosis were assessed in each group using an apoptosis assay based on Annexin V/propidium iodide staining. After 5 days in contact with the collagen matrix, the T cells expressed different surface markers. The overall T cell population increased significantly in the collagen matrix group compared to the respective controls (31.9±6.5 vs. 38.7±3.8%). CD8 and CD69 also increased significantly compared to their controls (CD69: 19.7±3.0 vs. 27.1±4.5% for collagen vs. control groups). At the same time, CD4 and CD38 expression was similar in both groups. Viability and apoptosis/necrosis were also identical in the samples and controls. These results show that the interaction between the collagen matrix and the immune cells stimulated activation of T cells and did not impair the healing process. AD - Department of Periodontology, ‘Victor Babes’ University of Medicine and Pharmacy, 300141 Timisoara, Romania Department of Restorative Dentistry and Endodontics, ‘Victor Babes’ University of Medicine and Pharmacy, 300141 Timisoara, Romania Department of Physiology, ‘Victor Babes’ University of Medicine and Pharmacy, 300230 Timisoara, Romania Department of Periodontology, University of Medicine and Pharmacy, 200349 Craiova, Romania Department of Periodontology, ‘Iuliu Hatieganu’ University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania Department of Fixed Prosthodontics, ‘Carol Davila’ University of Medicine and Pharmacy, 020021 Bucharest, Romania Department of Physiology, Faculty of Midwifery and Nursing, ‘Carol Davila’ University of Medicine and Pharmacy, 020021 Bucharest, Romania Department of Embryology, ‘Carol Davila’ University of Medicine and Pharmacy, 020021 Bucharest, Romania Department of Histology, ‘Victor Babes’ University of Medicine and Pharmacy, 300230 Timisoara, Romania Department of Biochemistry, Faculty of Dental Medicine, ‘Carol Davila’ University of Medicine and Pharmacy, 020021 Bucharest, Romania AU - Rusu,Darian AU - Boariu,Marius AU - Stratul,Ștefan-Ioan AU - Bojin,Florina AU - Paunescu,Virgil AU - Calniceanu,Horia AU - Surlin,Petra AU - Roman,Alexandra AU - Milicescu,Ștefan AU - Caruntu,Costin AU - Didilescu,Andreea AU - Gaje,Nela-Pusa AU - Calenic,Bogdan DA - 2019/02/01 DO - 10.3892/etm.2018.6979 EP - 996 IS - 2 JO - Exp Ther Med KW - T-lymphocytes oral mucosa collagen matrix apoptosis wound healing PY - 2019 SN - 1792-0981 1792-1015 SP - 990 ST - Interaction between a 3D collagen matrix used for periodontal soft tissue regeneration and T-lymphocytes: An in vitro pilot study T2 - Experimental and Therapeutic Medicine TI - Interaction between a 3D collagen matrix used for periodontal soft tissue regeneration and T-lymphocytes: An in vitro pilot study UR - https://doi.org/10.3892/etm.2018.6979 VL - 17 ER -