TY - JOUR AB - Wound healing process comprises a complex network of cells and molecules that are regulated in order to pursue tissue regeneration. Our study focused on the capacity of alveolar blood clots (ABCs), platelet-rich fibrin (PRF) and plasma rich in growth factors (PRGF) to induce in vitro fibroblasts proliferation and migration as a measure of alveolar regeneration. Using cellular impedance with xCELLigence technology we quantified the proliferation and the migration capacity of L929 fibroblast standard cell line in the presence of 4 different ABCs and 3 different PRFs harvested from healthy individuals during standard tooth extraction. We obtained a clear cellular proliferation induced by the compounds mainly after 24 h of cultivation, in a dose-dependent manner. After 48 h of cultivation we registered activated proliferation, but slightly decreased compared to the 24 h profile. Our data confirm that the presence of the blood clot is involved in the regenerative processes. The migratory capacity of fibroblasts was statistically activated by the PL compounds while not affected by the tested PRFs. The chemical mediators present within the blood clot, either produced by inflammatory cells captive within, or by endothelial or mesenchymal cells induced fibroblastic proliferation and subsequent collagen deposition. AD - Faculty of Dental Medicine, ‘Carol Davila’ University of Medicine and Pharmacy, 020021 Bucharest, Romania Department of Pathology, Colentina University Hospital, 020125 Bucharest, Romania AU - Bucur,Mihai AU - Constantin,Carolina AU - Neagu,Monica AU - Zurac,Sabina AU - Dinca,Octavian AU - Vladan,Cristian AU - Cioplea,Mirela AU - Popp,Cristiana AU - Nichita,Luciana AU - Ionescu,Ecaterina DA - 2019/02/01 DO - 10.3892/etm.2018.7063 EP - 989 IS - 2 JO - Exp Ther Med KW - fibroblasts cellular impedance alveolar blood clot platelet-rich fibrin plasma rich in growth factors tissue regeneration wound healing PY - 2019 SN - 1792-0981 1792-1015 SP - 982 ST - Alveolar blood clots and platelet-rich fibrin induce in vitro fibroblast proliferation and migration T2 - Experimental and Therapeutic Medicine TI - Alveolar blood clots and platelet-rich fibrin induce in vitro fibroblast proliferation and migration UR - https://doi.org/10.3892/etm.2018.7063 VL - 17 ER -