TY - JOUR AB - Sepsis commonly progresses to disseminated intravascular coagulation and induces the activation of heparanase (HPA) and the shedding of endothelial glycocalyx constituents, including syndecan‑1 (SDC‑1) and heparan sulphate (HS). However, the degradation of glycocalyx and its association with coagulation disorders remains undetermined. The present study aimed to evaluate the effect of unfractionated heparin (UFH) and N‑acetylheparin (NAH), which is a non‑anticoagulant heparin derivative, on endothelial glycocalyx and coagulation function in a lipopolysaccharide (LPS)‑induced sepsis rat model, and to compare the differences observed in coagulation function between UFH and NAH. Experimental rats were randomly assigned to four groups: Control; LPS; UFH + LPS; and NAH + LPS. Rats were administered UFH or NAH and subsequently, ~1 min later, administered LPS (10 mg/kg; intravenous). The blood and lung tissues of rats were collected 0.5, 2 and 6 h after LPS injection, and were used for subsequent analysis. The results demonstrated that HPA activity and SDC‑1 and HS levels increased, and this increase was associated with inflammatory cytokines and coagulation/fibrinolysis markers in the sepsis rat model. Histopathological examination was performed, and the lung injury score and lung wet/dry ratio indicated that UFH and NAH also significantly improved lung tissue injury. The results of the ELISA analysis demonstrated that UFH and NAH treatment: i) significantly decreased the levels of inflammatory cytokines including tumor necrosis factor‑α and interleukin‑6; ii) inhibited HPA activity and protected the integrity of the glycocalyx, which was identified by decreased HS and SDC‑1 levels; and iii) decreased the levels of prothrombin fragment 1+2, thrombin‑antithrombin complex, and plasminogen activator inhibitor‑1 and increased the levels of fibrinogen and antithrombin‑III. Preconditioning with UFH decreased the plasma activated partial thromboplastin time. These results indicated that UFH and NAH may alleviate sepsis‑induced coagulopathy, and this effect may have been due to an inhibition of HPA activity and decrease in the shedding of the endothelial glycocalyx. AD - Department of Respiratory Medicine and Intensive Care Unit, Binzhou Medical University Hospital, Binzhou, Shandong 256603, P.R. China Department of Cell Biology, Binzhou Medical University, Yantai, Shandong 264003, P.R. China Department of Clinical Laboratory, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, Shandong 264003, P.R. China AU - Huang,Xiao AU - Han,Shasha AU - Liu,Xiangyong AU - Wang,Tao AU - Xu,Haixiao AU - Xia,Bingyan AU - Kong,Guiqing AU - Li,Jiankui AU - Zhu,Weiwei AU - Hu,Haoran AU - Hao,Dong AU - Wang,Xiaozhi DA - 2020/02/01 DO - 10.3892/etm.2019.8285 EP - 922 IS - 2 JO - Exp Ther Med KW - sepsis unfractionated heparin N‑acetylheparin glycocalyx coagulation PY - 2020 SN - 1792-0981 1792-1015 SP - 913 ST - Both UFH and NAH alleviate shedding of endothelial glycocalyx and coagulopathy in LPS‑induced sepsis T2 - Experimental and Therapeutic Medicine TI - Both UFH and NAH alleviate shedding of endothelial glycocalyx and coagulopathy in LPS‑induced sepsis UR - https://doi.org/10.3892/etm.2019.8285 VL - 19 ER -