TY - JOUR AB - Lung adenocarcinoma accounts for a high proportion of lung cancers. Though efforts have been made to develop new and effective treatments for this disease, the mortality rate remains high. Gene expression microarrays facilitate the study of lung cancer at the molecular level. The present study aimed to detect differentially expressed protein‑coding genes to identify novel biomarkers and therapeutic targets for lung adenocarcinoma. Aberrations in gene expression in lung adenocarcinoma were determined by analysis of mRNA microarray datasets from the Gene Expression Omnibus database. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, protein‑protein interaction (PPI) networks and statistical analysis were used to identify the biological functions of the differentially expressed genes (DEGs). The results of the bioinformatics analysis were subsequently validated using reverse transcription‑quantitative PCR. A total of 303 DEGs were identified in lung adenocarcinomas, and they were enriched in a number of cancer‑associated GO terms and KEGG pathways. DNA topoisomerase 2 α (TOP2A), cell division cycle protein homolog 20 (CDC20), mitotic checkpoint serine/threonine protein kinase BUB1 (BUB1) and mitotic spindle assembly checkpoint protein MAD2A (MAD2L1) exhibited the highest degree of interaction in the PPI network. Survival analysis performed using Kaplan‑Meier curves and Cox regression indicated that these four genes were all significantly associated with the survival of patients with lung adenocarcinomas. In conclusion, TOP2A, CDC20, BUB1 and MAD2L1 may be key protein‑coding genes that may serve as biomarkers and therapeutic targets in lung adenocarcinomas. AD - Department of Pharmacology, School of Pharmacy, Qingdao University, Qingdao, Shandong 266000, P.R. China Department of Thoracic Surgery, Affiliated Hospital of Qingdao University, Qingdao, Shandong 266000, P.R. China AU - Wang,Luyao AU - Li,Shicheng AU - Wang,Yuanyong AU - Tang,Zhenxue AU - Liu,Chaolong AU - Jiao,Wenjie AU - Liu,Jia DA - 2020/02/01 DO - 10.3892/etm.2019.8300 EP - 1111 IS - 2 JO - Exp Ther Med KW - lung adenocarcinoma bioinformatics analysis biomarkers DNA topoisomerase 2α cell division cycle protein homolog 20 mitotic checkpoint serine/threonine protein kinase BUB1 mitotic spindle assembly checkpoint protein MAD2L1 PY - 2020 SN - 1792-0981 1792-1015 SP - 1103 ST - Identification of differentially expressed protein‑coding genes in lung adenocarcinomas T2 - Experimental and Therapeutic Medicine TI - Identification of differentially expressed protein‑coding genes in lung adenocarcinomas UR - https://doi.org/10.3892/etm.2019.8300 VL - 19 ER -