TY - JOUR AB - Staphylococcus aureus (S. aureus) is associated with recalcitrant chronic infection, especially in chronic rhinosinusitis (CRS). S. aureus infection and biofilms cause poorer postsurgical outcomes. We developed isosorbide mononitrate (ISMN) loaded nanoparticles conjugated with an anti‑Staphylococcus aureus alpha‑toxin (anti‑S. aureus α-toxin) antibody that could target biofilms and investigated their anti‑biofilm effect. Anti‑S. aureus α-toxin antibody coupled immunoliposomes were generated. The effect of ISMN immunoliposomes on S. aureus biofilm formation and their anti‑biofilm efficacy were examined using the crystal violet method and confocal laser scanning microscopy, respectively. Relative biofilm viability at 24 h was tested using the alamarBlue assay. The biofilm formation inhibitory effect on all concentrations of ISMN immunoliposomes was stronger than that of ISMN liposomes and free ISMN (P<0.05). At concentrations of 45 and 23 mg/ml, the inhibitory effect of ISMN liposomes was stronger than that of free ISMN (P<0.05), while at 11 mg/ml, the inhibitory effect of ISMN liposomes was the same as that of ISMN (P>0.05). At 45 and 23 mg/ml, the inhibitory effect of ISMN immunoliposomes on formed biofilms was greater than that of ISMN liposomes and free ISMN (P<0.05) and the inhibitory effect of ISMN liposomes was stronger than that of free ISMN (P<0.05). At 11 mg/ml, ISMN immunoliposomes, ISMN liposomes, and ISMN had the same effect on formed biofilms (P>0.05). In conclusion, ISMN immunoliposomes nearly completely destroy biofilm structure. ISMN immunoliposomes provide a promising approach for treating infectious diseases caused by S. aureus biofilms, including refractory CRS, chronic skin infection, sepsis, and osteomyelitis. AD - Department of Rhinology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China Department of Traditional Chinese Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China School of Pharmaceutical Science, Zhengzhou University, Zhengzhou, Henan 450052, P.R. China AU - Zhang,Yaqian AU - Zhao,Yulin AU - Dong,Dong AU - Li,Xiaoping AU - Li,Zhi AU - Li,Siyu AU - Wang,Juan DA - 2020/02/01 DO - 10.3892/etm.2019.8344 EP - 1274 IS - 2 JO - Exp Ther Med KW - chronic rhinosinusitis Staphylococcus aureus biofilm ISMN immunoliposomes nanoparticles PY - 2020 SN - 1792-0981 1792-1015 SP - 1267 ST - Effects of isosorbide mononitrate loaded nanoparticles conjugated with anti‑Staphylococcus aureus α‑toxin on Staphylococcus aureus biofilms T2 - Experimental and Therapeutic Medicine TI - Effects of isosorbide mononitrate loaded nanoparticles conjugated with anti‑Staphylococcus aureus α‑toxin on Staphylococcus aureus biofilms UR - https://doi.org/10.3892/etm.2019.8344 VL - 19 ER -