TY - JOUR AB - LipoxinA4 (LXA4) is a well‑known key mediator of endogenous anti‑inflammation and of the resolution of inflammation. Considerable oxidative stress occurs during inflammation due to the generation of reactive oxidative species (ROS). Moreover, high levels of uric acid (UA) contribute to endothelial cell dysfunction, which can promote disease‑related morbidity, and NADPH oxidase‑derived ROS are crucial regulatory factors in these responses. However, LXA4 also has the potential to reduce oxidative stress. The aim of the present study was to examine whether LXA4 could suppress UA‑induced oxidative stress in human umbilical vein endothelial cells (HUVECs) and to investigate its mechanisms of action in vitro. HUVECs were incubated with or without LXA4, followed by the addition of UA. ROS levels were then measured using 2,7‑dichlorodihydrofluorescein diacetate and lucigenin‑enhanced chemiluminescence was used to evaluate NADPH oxidase activity. p47phox or p22phox small interfering (si)RNA were transfected into HUVECs and protein levels of p47phox were detected using western blot analysis. LXA4 significantly inhibited UA‑induced generation of ROS to the same extent as the NADPH oxidase inhibitor, diphenyleneiodonium chloride. Notably, transfection of p47phox siRNA attenuated the generation of ROS and the activation of NADPH oxidase. Cells transfected with p22phox siRNA demonstrated a significant reduction in the expression of p47phox on the membrane. Further experiments demonstrated that LXA4 interfered with the transfer of p47phox from the cytoplasm to the cell membrane. These findings suggested that LXA4 inhibited the release of NADPH oxidase derived ROS in HUVECs stimulated by UA. A potential mechanism of action underlying this effect could be LXA4‑mediated suppression of NADPH oxidase activity, leading to inhibition of p47phox translocation from the cytoplasm to the cell membrane. AD - Department of Medical Laboratory, Central Hospital of Suining, Suining, Sichuan 629100, P.R. China Department of Ophthalmology, Central Hospital of Suining, Suining, Sichuan 629100, P.R. China Basic Medical Laboratory, Central Hospital of Suining, Suining, Sichuan 629100, P.R. China Department of Medical Laboratory, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China AU - Zhou,You AU - You,Hui AU - Zhang,Aijie AU - Jiang,Xingliang AU - Pu,Zheyan AU - Xu,Guoqiang AU - Zhao,Mingcai DA - 2020/08/01 DO - 10.3892/etm.2020.8812 EP - 1692 IS - 2 JO - Exp Ther Med KW - lipoxinA4 uric acid NADPH reactive oxidative species human umbilical vein endothelial cells PY - 2020 SN - 1792-0981 1792-1015 SP - 1682 ST - Lipoxin A4 attenuates uric acid‑activated, NADPH oxidase‑dependent oxidative stress by interfering with translocation of p47phox in human umbilical vein endothelial cells T2 - Experimental and Therapeutic Medicine TI - Lipoxin A4 attenuates uric acid‑activated, NADPH oxidase‑dependent oxidative stress by interfering with translocation of p47phox in human umbilical vein endothelial cells UR - https://doi.org/10.3892/etm.2020.8812 VL - 20 ER -