TY - JOUR AB - Porcine epidemic diarrhea virus (PEDV) has proven to be a major problem for the porcine industry worldwide. Conventional injectable vaccines induce effective systemic immune responses but are less effective in preventing PEDV at mucosal invasion sites, including the nasal or oral mucosa. Additionally, antigens delivered orally are easily degraded. Nasal immunization induces intestinal mucosal immune responses, which can aid in blocking viral invasion, and requires fewer antigen inoculation doses. Therefore, nasal immunizations are considered to be a potential approach to overcome viral infections. In the present study, nasal immunization of piglets was performed using inactivated PEDV combined with Bacillus subtilis as an immunopotentiator and the efficacy of nasal immunization was assessed. The results demonstrated that compared with oral immunization, piglets from the nasal immunization group exhibited higher levels of neutralizing antibodies (P<0.01) in the intestine, PEDV‑specific immunoglobulin (Ig)G (P<0.01) in serum and PEDV‑specific secretory IgA (SIgA) in saliva (P<0.01) and nasal secretions (P<0.01). An increased number of intestinal CD3+ T cells, IgA‑secreting cells and intraepithelial lymphocytes (P<0.05) were also observed. Furthermore, the protein expression levels of interleukin‑6 and interferon‑γ, relative to the control PEDV infection, were also significantly elevated (P<0.05). The results of the present study indicate that nasal immunization is more effective at inducing the intestinal mucosal immune response, and provide new insights into a novel vaccination strategy that may be used to decrease the incidence of PEDV infections. AD - MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, P.R. China AU - Zhang,En AU - Wang,Jialu AU - Li,Yuchen AU - Huang,Lulu AU - Wang,Yongheng AU - Yang,Qian DA - 2020/08/01 DO - 10.3892/etm.2020.8828 EP - 1606 IS - 2 JO - Exp Ther Med KW - porcine epidemic diarrhea virus vaccine nasal immunization oral immunization Bacillus subtilis PY - 2020 SN - 1792-0981 1792-1015 SP - 1596 ST - Comparison of oral and nasal immunization with inactivated porcine epidemic diarrhea virus on intestinal immunity in piglets T2 - Experimental and Therapeutic Medicine TI - Comparison of oral and nasal immunization with inactivated porcine epidemic diarrhea virus on intestinal immunity in piglets UR - https://doi.org/10.3892/etm.2020.8828 VL - 20 ER -