TY - JOUR AB - Compared with traditional imaging techniques, multimodal imaging obtains more accurate images that may increase disease detection rates. The present study prepared stromal cell‑derived factor 1 (SDF‑1)‑loaded, targeted nanoparticles coated with iron (II,III) oxide and perfluorohexane (PFH) to be used as polymer‑shelled contrast agents with multimodal imaging functions, with the aim of improving tongue cancer and lymph node metastasis diagnosis. The multifunctional, targeted, polymeric nanoparticles were prepared using a double emulsion method and chemokine SDF‑1 was conjugated to nanoparticles by a sulfide bond. The nanoparticles were spherical, uniform size and well dispersed. The results of the in vitro photoacoustic and ultrasonic imaging experiments demonstrated that the multifunctional nanoparticles displayed excellent multimodal imaging functions, as even small concentrations of nanoparticles presented clear ultrasound and photoacoustic imaging. When the temperature reached the boiling point of PFH (56˚C), a liquid‑gas phase change occurred and the microsphere volume and acoustic impedance increased, leading to enhanced ultrasonic development. The nanoparticles were automatically targeted to tongue squamous carcinoma cells in vitro via SDF‑1‑CXC chemokine receptor 4 interactions. The targeted experiment and flow cytometry results indicated that the nanoparticles underwent strong targeted binding to human tongue squamous cell carcinoma (SCC‑15) cells. In summary, the nanoparticles were automatically targeted to SCC‑15 cells and displayed promising characteristics for ultrasound and photoacoustic imaging. Higher concentrations of nanoparticles was associated with clearer imaged and greater echo intensity value and photoacoustic value. The present study established a foundation for the development of procedures for primary tongue cancer and lymph node metastasis diagnosis. AD - Depatment of Oral and Maxillofacial Surgery, Stomatological Hospital Affiliated to Chongqing Medical University, Chongqing 401147, P.R. China Chongqing Key Laboratory of Ultrasound Molecular Imaging, Chongqing Medical University, Chongqing 400010, P.R. China AU - Wang,Fei AU - Wang,Zhigang AU - Pang,Liang AU - Wan,Shuliang AU - Qiu,Lihua DA - 2020/09/01 DO - 10.3892/etm.2020.8925 EP - 2012 IS - 3 JO - Exp Ther Med KW - superparamagnetic iron oxide targeted nanoparticles contrast agent multimodal imaging tongue squamous cell carcinoma PY - 2020 SN - 1792-0981 1792-1015 SP - 2003 ST - Preparation and in vitro study of stromal cell‑derived factor 1‑targeted Fe3O4/poly(lactic‑co‑glycolic acid)/perfluorohexane nanoparticles T2 - Experimental and Therapeutic Medicine TI - Preparation and in vitro study of stromal cell‑derived factor 1‑targeted Fe3O4/poly(lactic‑co‑glycolic acid)/perfluorohexane nanoparticles UR - https://doi.org/10.3892/etm.2020.8925 VL - 20 ER -