TY - JOUR AB - The powerful ability of human amnion-derived mesenchymal stem cells (hAMSCs) to promote angiogenesis suggests that they may facilitate angiogenesis‑associated therapeutic strategies. However, the molecular mechanisms underlying hAMSC‑induced angiogenesis remain largely unknown. The present study results suggested that enhanced migration and tube formation in human umbilical vein endothelial cells (HUVECs) was induced by conditioned medium from hAMSCs (hAMSC‑CM). In addition, culture with this conditioned medium resulted in the increased expression of circular RNA ATP binding cassette subfamily B member 10 (circ‑ABCB10) and vascular endothelial growth factor A (VEGFA). In the present study genes related to thecirc‑ABCB10/microRNA (miR)‑29b‑3p/VEGFA pathway were predicted using bioinformatics software, and further investigated using in vitro luciferase reporter assays. Loss‑of‑function assays were performed using small interfering RNAs (siRNAs). The results suggested that siRNA‑silencing of circ‑ABCB10 in HUVECs weakened migration and tube formation of HUVECs following hAMSC‑CM treatment and reduced the levels of VEGFA expression. Treatment with an miR‑29b‑3p inhibitor could largely rescue these effects in HUVECs, following circ‑ABCB10 silencing. The present study results suggest that the circ‑ABCB10/miR‑29b‑3p/VEGFA pathway may be involved in the pro‑angiogenic role of hAMSC‑CM in HUVECs. AD - Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China Department of Anatomy, Histology and Embryology, Nanjing Medical University, Nanjing, Jiangsu 211100, P.R. China Department of Periodontology Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210029, P.R. China AU - Tang,Zichun AU - Tan,Junling AU - Yuan,Xiaoqin AU - Zhou,Qianwen AU - Yuan,Zhiyao AU - Chen,Ning AU - Shen,Ming DA - 2020/09/01 DO - 10.3892/etm.2020.8939 EP - 2030 IS - 3 JO - Exp Ther Med KW - circ‑ABCB10 hAMSCs angiogenesis HUVECs VEGFA PY - 2020 SN - 1792-0981 1792-1015 SP - 2021 ST - Circular RNA‑ABCB10 promotes angiogenesis induced by conditioned medium from human amnion‑derived mesenchymal stem cells via the microRNA‑29b‑3p/vascular endothelial growth factor A axis T2 - Experimental and Therapeutic Medicine TI - Circular RNA‑ABCB10 promotes angiogenesis induced by conditioned medium from human amnion‑derived mesenchymal stem cells via the microRNA‑29b‑3p/vascular endothelial growth factor A axis UR - https://doi.org/10.3892/etm.2020.8939 VL - 20 ER -