TY - JOUR AB - Long non‑coding RNAs (lncRNAs) Mirt2 and interferon‑γ antisense RNA I (IFNG‑AS1) play opposing roles in lipopolysaccharide (LPS)‑induced inflammation, a key initiator of ulcerative colitis (UC). The present study aimed to analyze the potential interaction between Mirt2 and IFNG‑AS1 in UC. Levels of IFNG‑AS1 and Mirt2 in plasma samples from UC patients were measured using reverse transcription‑quantitative PCR. Receiver operating characteristic curves were used to evaluate the diagnostic values of IFNG‑AS1 and Mirt2 fr UC. The role of Mirt2 and IFNG‑AS1 in colonic epithelial cell apoptosis was analyzed by cell apoptosis assay. In patients with UC, Mirt2 and IFNG‑AS1 exhibited an inverse correlation, in which Mirt2 was downregulated while IFNG‑AS1 was upregulated. Altered expression of IFNG‑AS1 and Mirt2 separated patients with UC from healthy controls. In colonic epithelial cells, lipopolysaccharide treatment led to the downregulation of Mirt2 and the upregulation of IFNG‑AS1. Furthermore, overexpression of Mirt2 in colonic epithelial cells resulted in downregulation of IFNG‑AS1, and vice versa. Overexpression of Mirt2 led to a decreased rate of colonic epithelial cell apoptosis, while overexpression of IFNG‑AS1 led to an increased rate of apoptosis. Moreover, IFNG‑AS1 overexpression attenuated the effects of Mirt2 overexpression. Therefore, Mirt2 may interact with IFNG‑AS1 during UC to participate in colonic epithelial cell apoptosis. AD - Department of Gastroenterology, Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China Department of Gastroenterology, Hainan General Hospital, Haikou, Hainan 570311, P.R. China AU - Li,Chenyang AU - Cui,Lujia AU - Li,Siqiong AU - Li,Minrui AU - Miao,Xinpu DA - 2020/11/01 DO - 10.3892/etm.2020.9159 IS - 5 JO - Exp Ther Med KW - Mirt2 interferon‑γ antisense RNA I ulcerative colitis apoptosis PY - 2020 SN - 1792-0981 1792-1015 SP - 32 ST - Long non‑coding RNA Mirt2 interacts with long non‑coding RNA IFNG‑AS1 to regulate ulcerative colitis T2 - Experimental and Therapeutic Medicine TI - Long non‑coding RNA Mirt2 interacts with long non‑coding RNA IFNG‑AS1 to regulate ulcerative colitis UR - https://doi.org/10.3892/etm.2020.9159 VL - 20 ER -