TY - JOUR AB - Cellular senescence decreases cell proliferation over time and is characterized by typical markers, including larger cell volume, a flattened morphology, irreversible cell cycle arrest, augmentation of senescence‑associated β‑galactosidase (SA‑β‑gal) activity and senescence‑associated secretory phenotype. A variety of factors are implicated in the process of cellular aging, which mediates an organisms' lifespan. Insulin‑like growth factor‑1 (IGF‑1) serves an essential role in regulating cell growth, division, proliferation and senescence. In the present study, the role of IGF‑1 and the downstream Akt signaling pathway in rat articular chondrocyte senescence was assessed. The results of the current study demonstrated that IGF‑1 promoted cellular senescence in rat articular chondrocytes via activation of SA‑β‑gal and the upregulation of p53 and p21 mRNA and protein levels. IGF‑1 enhanced Akt phosphorylation and treatment with Akt inhibitor, MK‑2206, significantly suppressed the induction of these markers. Overall, the results indicated the involvement of IGF‑1 and Akt in senescence exhibited by rat articular chondrocytes. AD - Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, P.R. China AU - Zhao,Li-Dong AU - Bie,Lian-Yu AU - Hu,Lan AU - Zhu,Zi-Han AU - Meng,Xing-Hua AU - Cong,Lin-Lin AU - Zhang,Shai AU - Ma,Ning AU - Xiao,Jian-Hua DA - 2020/11/01 DO - 10.3892/etm.2020.9177 IS - 5 JO - Exp Ther Med KW - insulin‑like growth factor‑1 senescence rat articular chondrocytes Akt PY - 2020 SN - 1792-0981 1792-1015 SP - 49 ST - IGF‑1 induces cellular senescence in rat articular chondrocytes via Akt pathway activation T2 - Experimental and Therapeutic Medicine TI - IGF‑1 induces cellular senescence in rat articular chondrocytes via Akt pathway activation UR - https://doi.org/10.3892/etm.2020.9177 VL - 20 ER -