TY - JOUR
AB - The aim of the present study was to investigate the effect and mechanism of action of microRNA (miR)‑27b on skin wound healing in rats with deep second‑degree scald burns and in BJ human skin fibroblast cells. Rat models with deep second‑degree scald burns were constructed and injected with miR‑27b mimics and inhibitors at the wound site daily for 21 days. Healing of burned skin tissues was observed at 0, 3, 7, 14 and 21 days following modeling. H&E and Masson staining were used to observe the pathological structure and degree of collagen fibers in the burned skin tissues. The effects of miR‑27b on BJ cell proliferation and migration were determined by MTT and scratch assays. Matrix metalloproteinase‑1 (MMP‑1), α‑smooth muscle actin (α‑SMA), collagen I and collagen III expression in rat skin tissues and BJ cells were measured via reverse transcription‑quantitative PCR and western blot analysis. The results of the in vivo experiments demonstrated that miR‑27b inhibition accelerated scalded skin healing and induced fibroblast growth. Furthermore, the in vitro experiments revealed that miR‑27b inhibition increased BJ cell proliferation and migration. Furthermore, miR‑27b inhibition upregulated MMP‑1, α‑SMA, collagen I and collagen III expression in the skin tissues and cells, while the overexpression of miR‑27b demonstrated the opposite effect. In conclusion, the results of the present study revealed that miR‑27b inhibition increased fibroblast proliferation, thereby accelerating scald wound healing in rats.
AD - Department of Burn and Cosmetology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China
AU - Bi,Qingxia
AU - Liu,Jingyan
AU - Wang,Xueming
AU - Sun,Furong
DA - 2020/11/01
DO - 10.3892/etm.2020.9191
IS - 5
JO - Exp Ther Med
KW - microRNA‑27b
deep second‑degree scald burn
fibroblasts
wound healing
PY - 2020
SN - 1792-0981
1792-1015
SP - 63
ST - Downregulation of miR‑27b promotes skin wound healing in a rat model of scald burn by promoting fibroblast proliferation
T2 - Experimental and Therapeutic Medicine
TI - Downregulation of miR‑27b promotes skin wound healing in a rat model of scald burn by promoting fibroblast proliferation
UR - https://doi.org/10.3892/etm.2020.9191
VL - 20
ER -