TY - JOUR AB - The aim of the present study was to investigate the effect and mechanism of action of microRNA (miR)‑27b on skin wound healing in rats with deep second‑degree scald burns and in BJ human skin fibroblast cells. Rat models with deep second‑degree scald burns were constructed and injected with miR‑27b mimics and inhibitors at the wound site daily for 21 days. Healing of burned skin tissues was observed at 0, 3, 7, 14 and 21 days following modeling. H&E and Masson staining were used to observe the pathological structure and degree of collagen fibers in the burned skin tissues. The effects of miR‑27b on BJ cell proliferation and migration were determined by MTT and scratch assays. Matrix metalloproteinase‑1 (MMP‑1), α‑smooth muscle actin (α‑SMA), collagen I and collagen III expression in rat skin tissues and BJ cells were measured via reverse transcription‑quantitative PCR and western blot analysis. The results of the in vivo experiments demonstrated that miR‑27b inhibition accelerated scalded skin healing and induced fibroblast growth. Furthermore, the in vitro experiments revealed that miR‑27b inhibition increased BJ cell proliferation and migration. Furthermore, miR‑27b inhibition upregulated MMP‑1, α‑SMA, collagen I and collagen III expression in the skin tissues and cells, while the overexpression of miR‑27b demonstrated the opposite effect. In conclusion, the results of the present study revealed that miR‑27b inhibition increased fibroblast proliferation, thereby accelerating scald wound healing in rats. AD - Department of Burn and Cosmetology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China AU - Bi,Qingxia AU - Liu,Jingyan AU - Wang,Xueming AU - Sun,Furong DA - 2020/11/01 DO - 10.3892/etm.2020.9191 IS - 5 JO - Exp Ther Med KW - microRNA‑27b deep second‑degree scald burn fibroblasts wound healing PY - 2020 SN - 1792-0981 1792-1015 SP - 63 ST - Downregulation of miR‑27b promotes skin wound healing in a rat model of scald burn by promoting fibroblast proliferation T2 - Experimental and Therapeutic Medicine TI - Downregulation of miR‑27b promotes skin wound healing in a rat model of scald burn by promoting fibroblast proliferation UR - https://doi.org/10.3892/etm.2020.9191 VL - 20 ER -