TY - JOUR
AB - The present study evaluated mRNA and long non‑coding RNA (lncRNA) expression profiles and the pathways involved in paroxysmal atrial fibrillation (ParoAF) and persistent atrial fibrillation (PersAF). Nine left atrial appendage (LAA) tissues collected from the hearts of patients with AF (patients with ParoAF=3; and patients with PersAF=3) and healthy donors (n=3) were analyzed by RNA sequencing. Differentially expressed (DE) mRNAs and lncRNAs were identified by |Log2 fold change|>2 and P<0.05. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes pathway enrichment, protein‑protein interaction network and mRNA‑lncRNA interaction network analyses of DE mRNA and mRNA at the upstream/downstream of DE lncRNA were conducted. A total of 285 and 275 DE mRNAs, 575 and 583 DE lncRNAs were detected in ParoAF and PersAF samples compared with controls, respectively. PI3K/Akt and transforming growth factor‑β signaling pathways were significantly enriched in the ParoAF_Control and the calcium signaling pathway was significantly enriched in the PersAF_Control. Cis and trans analyses revealed some important interactions in DE mRNAs and lncRNA, including an interaction of GPC‑AS2 with dopachrome tautomerase, and phosphodiesterase 4D and cAMP‑specific with XLOC_110310 and XLOC_137634. Overall, the present study provides a molecular basis for future clinical studies on ParoAF and PersAF.
AD - Department of Cardiovascular Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China
AU - Sun,Haoliang
AU - Shao,Yongfeng
DA - 2021/06/01
DO - 10.3892/etm.2021.10003
IS - 6
JO - Exp Ther Med
KW - paroxysmal atrial fibrillation
persistent atrial fibrillation
RNA sequencing
Kyoto Encyclopedia of Genes and Genomes pathway
mRNA
long non‑coding RNA
PY - 2021
SN - 1792-0981
1792-1015
SP - 571
ST - Transcriptome analysis reveals key pathways that vary in patients with paroxysmal and persistent atrial fibrillation
T2 - Experimental and Therapeutic Medicine
TI - Transcriptome analysis reveals key pathways that vary in patients with paroxysmal and persistent atrial fibrillation
UR - https://doi.org/10.3892/etm.2021.10003
VL - 21
ER -