TY - JOUR AB - Plantamajoside (PMS), a major component of Plantago asiatica L, has several pharmacological properties, including anti‑proliferative, anti‑inflammatory and anti‑tumor effects. However, the effects of PMS on hepatocellular carcinoma (HCC) have yet to be determined. The aim of the present study was to investigate the effects of PMS on HCC and elucidate the underlying mechanism. All assays were conducted using 5 groups, namely control, sorafenib, and PMS 100, 50, and 25 µg/ml groups. Cell proliferation was determined by the MTT assay. Cell migration was evaluated with the wound healing and Transwell assays, respectively. Cell apoptosis and cell cycle distribution were evaluated via flow cytometry. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analysis and western blotting were used to further investigate the mechanism of action of PMS. Sorafenib and PMS both significantly attenuated the proliferation and migration of HCC cells, and markedly promoted cell apoptosis. PMS induced cell cycle arrest in the G0/G1 phase. The efficacy of PMS increased in a dose‑dependent manner. Further study evaluated the expression of peroxisome proliferator‑activated receptor (PPARγ), nuclear factor (NF)‑κB and cyclooxygenase (Cox‑2) using RT‑qPCR analysis and western blotting. The results demonstrated that PMS promoted the expression of PPARγ and suppressed the expression of NF‑κB and Cox‑2. In conclusion, PMS was shown to affect cell proliferation, migration, apoptosis and cell cycle distribution. Furthermore, PMS promoted the expression of PPARγ and inhibited the expression of NF‑κB and Cox‑2, which may be the mechanism underlying its biological effects. Based on the results of the present study, PMS appears to be a promising agent for HCC therapy. AD - Department of Traditional Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, P.R. China Department of Nursing, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, P.R. China Department of Science and Technology, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, P.R. China Department of Gastroenterology, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, P.R. China Department of Traditional Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023 Central Laboratory, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, P.R. China AU - Luo,Shu AU - Jiang,Xing AU - Yin,Gang AU - Liu,Yajun AU - Liu,Zhou AU - Meng,Linglian AU - Wu,Jian AU - Wu,Haoxin DA - 2021/06/01 DO - 10.3892/etm.2021.10005 IS - 6 JO - Exp Ther Med KW - plantamajoside hepatocellular carcinoma herbal medicine peroxisome proliferator‑activated receptor γ PY - 2021 SN - 1792-0981 1792-1015 SP - 573 ST - The herbal agent plantamajoside, exerts a potential inhibitory effect on the development of hepatocellular carcinoma T2 - Experimental and Therapeutic Medicine TI - The herbal agent plantamajoside, exerts a potential inhibitory effect on the development of hepatocellular carcinoma UR - https://doi.org/10.3892/etm.2021.10005 VL - 21 ER -