TY - JOUR AB - The NICE‑3 protein serves an oncogenic role in hepatocellular carcinoma, but its role in lung adenocarcinoma (LUAD) remains unknown. The aim of the present study was to investigate the potential role and underlying mechanisms of NICE‑3 in LUAD. In the present study, NICE‑3 expression in LUAD tissues and its association with patient prognosis were analyzed using datasets from The Cancer Genome Atlas and Gene Express Omnibus. After NICE‑3‑knockdown with small interfering RNA in LUAD cells, cell proliferation was measured by cell counting, cell cycle was examined by flow cytometry, cell invasion and migration were detected by Transwell assays and autophagic markers LC3 and p62, as well as phosphorylation of S6K and AKT, were determined by western blotting. The results of public database analysis demonstrated that compared with normal lung tissues, NICE‑3 expression was increased in LUAD tissues, where high expression levels were associated with a poor prognosis. The results of in vitro experimentation in LUAD cells indicated that NICE‑3‑knockdown inhibited proliferation, cell cycle, migration and invasion, but enhanced autophagy. Notably, NICE‑3‑knockdown inhibited AKT/mTORC1 signaling. The present results suggested that NICE‑3 may serve an oncogenic role in LUAD via the AKT/mTORC1 signaling pathway and may therefore be a potential therapeutic target for LUAD. AD - Laboratory of Respiratory Diseases, The Affiliated Hospital of Guilin Medical University, Guilin, Guangxi 541001, P.R. China AU - Du,Longxia AU - Wu,Youru AU - Han,Xiaodan AU - Wang,Chen AU - Li,Aili AU - Huang,Guojin DA - 2021/06/01 DO - 10.3892/etm.2021.10057 IS - 6 JO - Exp Ther Med KW - NICE‑3 chromosome 1 open reading frame 43 lung adenocarcinoma proliferation cell cycle autophagy PY - 2021 SN - 1792-0981 1792-1015 SP - 625 ST - NICE‑3‑knockdown induces cell cycle arrest and autophagy in lung adenocarcinoma cells via the AKT/mTORC1 signaling pathway T2 - Experimental and Therapeutic Medicine TI - NICE‑3‑knockdown induces cell cycle arrest and autophagy in lung adenocarcinoma cells via the AKT/mTORC1 signaling pathway UR - https://doi.org/10.3892/etm.2021.10057 VL - 21 ER -