TY - JOUR AB - Hypothermic treatment is known to protect against cardiac arrest (CA) and improve survival rate. However, few studies have evaluated the CA‑induced liver damage and the effects of hypothermia on this damage. Therefore, the aim of the present study was to determine possible protective effects of hypothermia on the liver after asphyxial CA. Rats were subjected to a 5‑min asphyxial CA followed by return of spontaneous circulation (ROSC). The body temperature was controlled at 37±0.5˚C (normothermia group) or 33±0.5˚C (hypothermia group) for 4 h after ROSC. Livers were examined at 6, 12 h, 1 and 2 days after ROSC. Histopathological examination was performed by H&E staining. Alterations in the expression levels of pro‑inflammatory (TNF‑α and interleukin IL‑2) and anti‑inflammatory cytokines (IL‑4 and IL‑13) were investigated by immunohistochemistry. Sinusoidal dilatation and vacuolization were observed after asphyxial CA by histopathological examination. However, these CA‑induced structural alterations were prevented by hypothermia. In immunohistochemical examination, the expression levels of pro‑inflammatory cytokines were reduced in the hypothermia group compared with those in the normothermia group while the expression levels of anti‑inflammatory cytokines were increased in the hypothermia group compared with those in the normothermia group. In conclusion, hypothermic treatment for 4 h following asphyxial CA in rats inhibited the increase of pro‑inflammatory cytokines and stimulated the expression of anti‑inflammatory cytokines compared with the normothermic group. The results of the present study suggested that hypothermic treatment after asphyxial CA reduced liver damage via the regulation of inflammation. AD - Department of Emergency Medicine, Kangwon National University Hospital, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24289, Republic of Korea Department of Physical Therapy, College of Health Science, Youngsan University, Yangsan, Gyeongnam 50510, Republic of Korea Bio‑Safety Research Institute, College of Veterinary Medicine, Chonbuk National University, Iksan, Jeollabuk 54596, Republic of Korea Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chuncheon, Gangwon 24252, Republic of Korea Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea Department of Anatomy, College of Korean Medicine, Dongguk University, Gyeongju, Gyeongbuk 38066, Republic of Korea AU - Park,Yoonsoo AU - Ahn,Ji,Hyeon AU - Cho,Jeong,Hwi AU - Tae,Hyun-Jin AU - Lee,Tae-Kyeong AU - Kim,Bora AU - Lee,Jae-Chul AU - Park,Joon,Ha AU - Shin,Myoung,Cheol AU - Ohk,Taek,Geun AU - Cho,Jun,Hwi AU - Won,Moo-Ho DA - 2021/06/01 DO - 10.3892/etm.2021.10058 IS - 6 JO - Exp Ther Med KW - anti‑inflammatory cytokines asphyxial cardiac arrest hypothermia liver pro‑inflammatory cytokines PY - 2021 SN - 1792-0981 1792-1015 SP - 626 ST - Effects of hypothermia on inflammatory cytokine expression in rat liver following asphyxial cardiac arrest T2 - Experimental and Therapeutic Medicine TI - Effects of hypothermia on inflammatory cytokine expression in rat liver following asphyxial cardiac arrest UR - https://doi.org/10.3892/etm.2021.10058 VL - 21 ER -