TY - JOUR
AB - Kallikrein‑related peptidase 6 (KLK6), a member of the kallikrein‑related peptidase family, is involved in the regulation of epithelial‑mesenchymal transition (EMT) in cancer cells and is highly expressed in gastric cancer tissues. The aim of the present study was to investigate the effect of KLK6 on the proliferation, migration and invasion of gastric cancer cells and to determine the underlying mechanism of its actions. The expression of KLK6 was measured in metastatic gastric cancer cells using western blotting and reverse transcription‑quantitative PCR, and KLK6 was overexpressed or inhibited in HGC‑27 cells using plasmid transfection. Cell proliferation, migration, invasion and EMT were also evaluated using Cell Counting Kit 8, Transwell and western blot analysis, respectively. In addition, a mouse xenograft model was constructed by injection of HGC‑27 cells. The xenograft was treated with KLK6 interference or overexpression plasmids to study the in vivo effects of KLK6 on tumor development. The results demonstrated that KLK6 was highly expressed in HGC‑27 cells and that KLK6 inhibition attenuated cell proliferation, migration and invasion and prevented gastric cancer tumor development. In addition, KLK6 inhibition reduced the expression of epithelial cell adhesion molecule and vimentin, reduced the phosphorylation of SMAD2 and SMAD3 and upregulated epithelial‑cadherin expression. In conclusion, KLK6 inhibition suppressed the proliferation, migration and invasion of gastric cancer cells both in vitro and in vivo through the inhibition of EMT. These findings indicate that KLK6 a potential therapeutic target for gastric cancer therapy.
AD - Department of Vascular Surgery, No. 1 People's Hospital, Hubei University of Medicine, Xiangyang, Hubei 441000, P.R. China
AU - Zhou,Dong
AU - He,Yanping
AU - Li,Hengping
AU - Huang,Weidong
DA - 2021/07/01
DO - 10.3892/etm.2021.10202
IS - 1
JO - Exp Ther Med
KW - gastric cancer
kallikrein‑related peptidase 6
migration
invasion
epithelial‑mesenchymal transition
PY - 2021
SN - 1792-0981
1792-1015
SP - 770
ST - Silencing of kallikrein‑related peptidase 6 attenuates the proliferation, migration, and invasion of gastric cancer cells through inhibition of epithelial‑mesenchymal transition
T2 - Experimental and Therapeutic Medicine
TI - Silencing of kallikrein‑related peptidase 6 attenuates the proliferation, migration, and invasion of gastric cancer cells through inhibition of epithelial‑mesenchymal transition
UR - https://doi.org/10.3892/etm.2021.10202
VL - 22
ER -