TY - JOUR
AB - Suspicion of lead poisoning is confirmed by its concentration in blood and protoporphyrin red blood cells. At low concentrations, lead influences the synthesis of the heme in the sense of lowering it. Acute and chronic lead intoxication is extremely polymorphic in regards to its clinical manifestations, with digestive, hematological, cardiovascular, renal hepatic and neurological features. The aim of the study was to evaluate the presence of lead in human whole blood and urine harvested before and during chelation treatment in the case of lead poisoning. An atomic absorption spectroscopic method for the analysis of lead was developed using graphite furnace atomic absorption spectrophotometer (GF‑AAS), Varian Spectra AA‑880 with a hollow cathode lead lamp and a deuterium lamp for background correction, coupled to a GTA‑100 atomizer and a programmable sample dispenser. Standard calibration solutions were used for the range 10‑100 µg/l. The linearity range was 10.0 to 100.0 µg/l with the correlation coefficient of 0.999. We established that the method can be applied for the determination of lead in whole blood and urine, and the results obtained are useful for monitoring chelation therapy in cases of acute lead poisoning, a neglected cause of abdominal colic pain in an emergency situation.
AD - Faculty of Medicine, ‘Carol Davila’ University of Medicine and Pharmacy, 020011 Bucharest, Romania
Military Medical Research Center, 010919 Bucharest, Romania
Faculty of Medicine, The University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania
Fourth Surgery Department, Emergency University Hospital Bucharest, 050098 Bucharest, Romania
Third Department, Faculty of Medicine, ‘Lucian Blaga’ University of Sibiu, 550169 Sibiu, Romania
Faculty of Medicine, Ovidius University, 900470 Constanta, Romania
AU - Tudosie,Mihail,Silviu
AU - Caragea,Genica
AU - Popescu,Dragos,Marian
AU - Avram,Oana
AU - Serban,Dragos
AU - Smarandache,Catalin,Gabriel
AU - Tudor,Corneliu
AU - Badiu,Cristinel,Dumitru
AU - Socea,Bogdan
AU - Dan Sabau,Alexandru
AU - Comandasu,Meda
AU - Spataru,Radu
AU - Costea,Daniel,Ovidiu
AU - Tanasescu,Ciprian
AU - Dascalu,Ana,Maria
DA - 2021/09/01
DO - 10.3892/etm.2021.10417
IS - 3
JO - Exp Ther Med
KW - lead toxicity
GF‑AAS
biological samples
saturnine colic
acute abdominal pain
PY - 2021
SN - 1792-0981
1792-1015
SP - 985
ST - Optimization of a GF‑AAS method for lead testing in blood and urine: A useful tool in acute abdominal pain management in emergency
T2 - Experimental and Therapeutic Medicine
TI - Optimization of a GF‑AAS method for lead testing in blood and urine: A useful tool in acute abdominal pain management in emergency
UR - https://doi.org/10.3892/etm.2021.10417
VL - 22
ER -