TY - JOUR AB - DL‑3‑n‑butylphthalide (NBP) is commonly used to treat ischemic strokes due to its antioxidative and anti‑inflammatory effects. The present study aimed to examine the protective effects of NBP on myocardial ischemia‑reperfusion injury (MIRI) by establishing a MIRI model in H9c2 cells. Cell viability assay using Cell Counting Kit‑8, lactate dehydrogenase (LDH) cytotoxicity and lipid peroxidation malondialdehyde (MDA) content were assessed to detect cell activity, degree of cell injury and oxidative stress reaction. Reverse transcription‑quantitative PCR was used to quantify the expression of inflammatory factors in H9c2 cells. Western blotting and immunofluorescence staining were used to detect the protein expression of PI3K/AKT and heat shock protein 70 (HSP70). The present results indicated that NBP significantly increased cell viability during ischemia‑reperfusion. Moreover, NBP inhibited the release of LDH and the production of MDA. NBP treatment also significantly decreased the expression of inflammatory factors at the mRNA level. Additionally, NBP activated the PI3K/AKT pathway and upregulated the expression of HSP70 compared with cells in the MIRI model. LY294002, a PI3K inhibitor, reversed the protective effects of NBP and suppressed the expression of HSP70. The present study demonstrated that NBP protected H9c2 cells from MIRI by regulating HSP70 expression via PI3K/AKT pathway activation. AD - Department of Cardiovascular Surgery, Affiliated Hospital of Weifang Medical University, Weifang, Shandong 261000, P.R. China School of Anesthesiology, Shandong Provincial Medicine and Health Key Laboratory of Clinical Anesthesia, Weifang Medical University, Weifang, Shandong 261000, P.R. China Department of Endocrinology, Affiliated Hospital of Weifang Medical University, Weifang, Shandong 261000, P.R. China AU - Yu,Yunchen AU - Zhu,Yuying AU - Sun,Xiaotong AU - Li,Yongxing AU - Wang,Mingling AU - Dong,Bin AU - Sun,Xiaodong AU - Hou,Wenming DA - 2021/09/01 DO - 10.3892/etm.2021.10441 IS - 3 JO - Exp Ther Med KW - dl‑3‑n‑butylphthalide myocardial ischemia‑reperfusion injury PI3K/AKT heat shock protein 70 H9c2 cells PY - 2021 SN - 1792-0981 1792-1015 SP - 1008 ST - DL‑3‑n‑butylphthalide protects H9c2 cardiomyoblasts from ischemia/reperfusion injury by regulating HSP70 expression via PI3K/AKT pathway activation T2 - Experimental and Therapeutic Medicine TI - DL‑3‑n‑butylphthalide protects H9c2 cardiomyoblasts from ischemia/reperfusion injury by regulating HSP70 expression via PI3K/AKT pathway activation UR - https://doi.org/10.3892/etm.2021.10441 VL - 22 ER -