TY - JOUR AB - Associations between gene variations and sudden cardiac arrest or coronary artery disease have been reported by genome‑wide association studies. However, the implication of the genetic status in cases of sudden coronary death (SCD) from the Chinese Han population has remained to be investigated. The present study established a mini‑sequencing system to examine putative death‑causing single nucleotide polymorphisms (SNPs) using multiplex PCR, single base extension reaction and capillary electrophoresis techniques. A total of 198 samples from the Chinese Han population (age range, 34‑71 years; mean age, 53.86 years) were examined using this method. Samples were classified into three groups: Coronary heart disease (CHD, n=70), SCD (n=53) and control (n=75) group. Significant associations were identified for 10, 4 and 6 SNPs in CHD, SCD and sudden death from CHD, respectively, using the χ2 test. The SNPs obtained by binary logistic regression may be used to assess and predict the risk of disease. The predictive accuracy of the SNPs in each prediction model and their area under the receiver operating characteristic curve (AUC) values were determined. The AUC of the four SNPs (rs12429889, rs10829156, rs16942421 and rs12155623) to predict CHD was 0.928, the AUC of the six SNPs (rs2389202, rs2982694, rs10183640, rs597503, rs16942421 and rs12155623) to predict SCD was 0.922 and the AUC of the four SNPs (rs16866933, rs4621553, rs10829156 and rs12155623) to predict sudden death from CHD was 0.912. The multifactor dimensionality reduction values were as follows: 0.8690 (prediction model of CHD), 0.7601 (prediction model of SCD) and 0.7628 (prediction model of sudden death from CHD). Taken together, the results of the present study suggested that these SNPs have considerable potential for application in genetic tests to predict CHD or SCD. However, further studies are required to investigate the putative functions of these SNPs. AD - Department of Clinical Laboratory and Pathology, Municipal Key‑Innovative Discipline of Molecular Diagnostics, Jiaxing Hospital of Traditional Chinese Medicine, Jiaxing University, Jiaxing, Zhejiang 314001, P.R. China Department of Cardiovascular Medicine, Fenyang Hospital of Shanxi Province, Fenyang Hospital Affiliated to Shanxi Medical University, Fenyang, Shanxi 032200, P.R. China Department of Forensic Biology, School of Forensic Medicine, Shanxi Medical University, Jinzhong, Shanxi 030619, P.R. China Department of Pathology, Forensic and Pathology Laboratory, Judicial Expertise Center, Jiaxing University Medical College, Jiaxing, Zhejiang 314001, P.R. China AU - Zhang,Nenghua AU - Lv,Xiaochun AU - Cheng,Xiaojuan AU - Wang,Jiaqi AU - Liu,Jinding AU - Shi,Jie AU - Liu,Jie AU - Hu,Bo AU - Chen,Deqing AU - Zhang,Gengqian DA - 2021/10/01 DO - 10.3892/etm.2021.10502 IS - 4 JO - Exp Ther Med KW - sudden coronary death SNaPshot single nucleotide polymorphism coronary heart disease capillary electrophoresis PY - 2021 SN - 1792-0981 1792-1015 SP - 1068 ST - Risk of sudden coronary death based on genetic background in Chinese Han population T2 - Experimental and Therapeutic Medicine TI - Risk of sudden coronary death based on genetic background in Chinese Han population UR - https://doi.org/10.3892/etm.2021.10502 VL - 22 ER -