TY - JOUR AB - No prospective study has specifically examined the serum levels of matrix metalloproteinase‑9 (MMP‑9) and tissue inhibitor of metalloproteinase‑1 (TIMP‑1) in the early shock phase of burn‑injured patients. Thus, we aimed to detect early changes, activity dynamics, and the predictive value of MMP‑9, TIMP‑1, and the MMP‑9/TIMP‑1 ratio to better understand the early repair mechanisms for the development of future therapies for patients with thermal burns. Twenty‑five patients with a total body surface area (TBSA) affected by burn <25%, and 30 healthy subjects were enrolled in the study. Serum levels of MMP‑9 and TIMP‑1 were determined by the ELISA method. Our results showed that MMP‑9 concentrations increased immediately after injury and remained on a plateau. In contrast, TIMP‑1 showed an upward trend throughout the 7‑day study period, and the time course of the MMP‑9/TIMP‑1 ratio followed the inverse dynamics of TIMP‑1. Analysis of the areas under the receiver operating characteristic (ROC) curves (AUC) showed that patients with burn wounds tended to have a MMP‑9 value higher than 421.5 ng/ml (AUC=0.979), TIMP‑1 value higher than 231.6 ng/ml (AUC=0.908), and MMP‑9/TIMP‑1 ratio higher than 2.31 (AUC=0.959) (P<0.001). Our findings suggest that although the variations in the two biomarkers were different regarding the time of the initial insult, their ratio is a specific and sensitive indicator of burn evolutivity in patients with a TBSA affected by a burn <25%. AD - Department of Carcinogenesis and Molecular Biology, ‘Prof. Dr. Alexandru Trestioreanu’ Institute of Oncology, 022328 Bucharest, Romania ENT Department, ‘Grigore Alexandrescu’ Children's Emergency Hospital, 011743 Bucharest, Romania Electrical Engineering Faculty, ‘Politehnica’ University, 060042, Bucharest, Romania Department of Nuclear Medicine, ‘Prof. Dr. Alexandru Trestioreanu’ Institute of Oncology, 022328 Bucharest, Romania ENT Department, ‘Prof. Dr. Dorin Hociota’ Institute of Phonoaudiology and Functional ENT and Cervicofacial Surgery, 050751 Bucharest, Romania Department of Complementary Sciences, ‘Carol Davila’ University of Medicine and Pharmacy, 050463 Bucharest, Romania ENT Department, ‘Carol Davila’ University of Medicine and Pharmacy, 050463 Bucharest, Romania AU - Stanciu,Adina,Elena AU - Zamfir‑Chiru‑Anton,Adina AU - Stanciu,Marcel,Marian AU - Gherghe,Mirela AU - Hainarosie,Razvan AU - Furtunescu,Florentina,Ligia AU - Gheorghe,Dan,Cristian DA - 2021/10/01 DO - 10.3892/etm.2021.10547 IS - 4 JO - Exp Ther Med KW - burn injury MMP‑9 TIMP‑1 MMP‑9/TIMP‑1 ratio ROC curve PY - 2021 SN - 1792-0981 1792-1015 SP - 1113 ST - Clinical significance of serum matrix metalloproteinase 9 and tissue inhibitor of metalloproteinase 1 in the first phase of burn trauma evolution T2 - Experimental and Therapeutic Medicine TI - Clinical significance of serum matrix metalloproteinase 9 and tissue inhibitor of metalloproteinase 1 in the first phase of burn trauma evolution UR - https://doi.org/10.3892/etm.2021.10547 VL - 22 ER -