TY - JOUR
AB - Liver cancer is one of the most common malignancies worldwide and poses a serious threat to human health. The most important treatment method, liver cancer chemotherapy, is limited due to its high toxicity and poor specificity. Targeted drug delivery systems have emerged as novel therapeutic strategies that deliver precise, substantial drug doses to target sites via targeting vectors and enhance the therapeutic efficacy. In the present study, glycyrrhetinic acid‑modified hyaluronic acid (GA‑HA) was used as a carrier for the model drug docetaxel (DTX) to prepare DTX‑loaded GA‑HA nanoparticles (DTX/GA‑HA‑NPs). The results indicated that the DTX/GA‑HA‑NPs exhibited high monodispersity (particle dispersity index, 0.209±0.116) and desirable particle size (208.73±5.0 nm) and zeta potential (‑27.83±3.14 mV). The drug loading capacity and encapsulation efficiency of the NPs were 12.59±0.68 and 85.38±4.62%, respectively. Furthermore, it was determined that FITC‑GA‑HA was taken up by cells and distributed in the cytoplasm. DTX and DTX/GA‑HA (just the DTX delivered by the nanoparticle) aggregated and altered the structure of cellular microtubules. Compared with DTX alone, DTX/GA‑HA‑NPs had a stronger inhibitory effect on HepG2 cell proliferation and promoted apoptosis of HepG2 cells. All experimental results indicated that DTX/GA‑HA‑NPs were successfully prepared and had liver‑targeting and antitumor activities in vitro, which provided a foundation for future in vivo studies of the antitumor effects of DTX/GA‑HA‑NPs.
AD - Shandong Key Laboratory of Medical and Health Sciences, Key Laboratory of Biotechnological Medicine in Universities of Shandong, School of Bioscience and Technology, Weifang Medical University, Weifang, Shandong 261053, P.R. China
Department of Pharmacology, Laboratory of Applied Pharmacology, College of Pharmacy, Weifang Medical University, Weifang, Shandong 261053, P.R. China
AU - Xue,Hantao
AU - Qin,Liya
AU - Zhang,Longxiang
AU - Li,Xiaocheng
AU - Wu,Fei
AU - Wang,Weiyu
AU - Wang,Chen
AU - Diao,Wenbin
AU - Jiang,Bin
AU - Lian,Bo
AU - Wu,Jingliang
AU - Bai,Jingkun
AU - Sun,Tongyi
AU - Zhao,Chunling
AU - Qu,Meihua
AU - Yu,Wenjing
AU - Wang,Yubing
AU - Gao,Zhiqin
DA - 2021/10/01
DO - 10.3892/etm.2021.10578
IS - 4
JO - Exp Ther Med
KW - glycyrrhetinic acid
hyaluronic acid
docetaxel
nanoparticles
microtubules
cytotoxicity
PY - 2021
SN - 1792-0981
1792-1015
SP - 1144
ST - Preparation of docetaxel‑loaded, glycyrrhetinic acid‑modified nanoparticles and their liver‑targeting and antitumor activity
T2 - Experimental and Therapeutic Medicine
TI - Preparation of docetaxel‑loaded, glycyrrhetinic acid‑modified nanoparticles and their liver‑targeting and antitumor activity
UR - https://doi.org/10.3892/etm.2021.10578
VL - 22
ER -