TY - JOUR
AB - Cardiac fibrosis is a core process in the development of heart failure. However, the underlying mechanism of cardiac fibrosis remains unclear. Recently, a study found that in an isoproterenol (ISO)‑induced cardiac fibrosis animal model, there is high expression of latent‑transforming growth factor β‑binding protein 2 (LTBP2) in cardiac fibroblasts. Whether LTBP2 serves a role in cardiac fibrosis is currently unknown. In the present study, mouse cardiac fibroblasts (MCFs) were treated with 100 µM/l ISO for 24, 48, or 72 h, and small interfering RNAs (siRNAs) were used to knockdown LTBP2. Reverse transcription‑quantitative PCR and western blotting were used to determine gene and protein expression levels, respectively. Caspase‑3 serves a key role in cell apoptosis and is related to cardiac fibrosis‑induced heart failure. Caspase‑3 activity was therefore determined using a caspase‑3 assay kit, CCK8 was used to determine the rate of cell proliferation and apoptosis rates were quantified using a cell death detection ELISA kit. The present study demonstrated that cell apoptosis and LTBP2 expression increased in MCFs treated with 100 µM/l ISO in a time‑dependent manner. Expression and activity of caspase‑3 also increased in MCFs treated with 100 µM/l ISO for 48 h compared with the control group. In addition, ISO stimulation‑induced MCF apoptosis, along with the increased expression of caspase‑3 were partly abolished when LTBP2 was knocked down. In conclusion, LTBP2 expression increased in ISO‑treated MCFs and accelerated mouse cardiac fibroblast apoptosis by enhancing the expression and activity of caspase‑3. LTBP2 may therefore be a potential therapeutic target for treating patients with cardiac fibrosis.
AD - Cardiovascular Department, Jinqiu Hospital of Liaoning Province, Shenyang, Liaoning 110016, P.R. China
AU - Shi,Chunlai
AU - Li,Xuan
AU - Hong,Fei
AU - Wang,Xueyan
AU - Jiang,Tingting
AU - Sun,Bingbing
AU - Li,Shuang
DA - 2021/10/01
DO - 10.3892/etm.2021.10580
IS - 4
JO - Exp Ther Med
KW - myocardial remodeling
latent‑transforming growth factor β‑binding protein 2
mouse cardiac fibroblasts
caspase‑3
apoptosis
PY - 2021
SN - 1792-0981
1792-1015
SP - 1146
ST - Latent‑transforming growth factor β‑binding protein 2 accelerates cardiac fibroblast apoptosis by regulating the expression and activity of caspase‑3
T2 - Experimental and Therapeutic Medicine
TI - Latent‑transforming growth factor β‑binding protein 2 accelerates cardiac fibroblast apoptosis by regulating the expression and activity of caspase‑3
UR - https://doi.org/10.3892/etm.2021.10580
VL - 22
ER -