TY - JOUR AB - Kidney disease represents a burden for the health care system worldwide. As the prevalence continues to rise, discovering new biomarkers of early kidney damage has become crucial. Oxidative stress (OS) represents one of the main factors involved in the early stages of many syndromes leading to kidney damage. Therefore, it must be studied in detail. To date, many studies have focused on OS in advanced stages of acute kidney injury (AKI), with great success. The aim of the present study was to ascertain whether even mild renal function impairment can be linked to specific systemic markers of OS and systemic antioxidants in order to pinpoint certain biomarkers for early kidney damage. We used male rats (Rattus norvegicus) in which we induced kidney damage by injecting gentamicin for 7 days. Blood was collected 24 h after the last dose of gentamicin. Urea, creatinine, 3‑nitrotyrosine (3‑NT), nitric oxide (NO), malondialdehyde (MDA), thiols (TS), total oxidative stress (TOS), and interferon‑γ (IFN‑γ) were determined. In addition, for the antioxidant status we measured total antioxidant capacity (TAC) and interleukin‑10 (IL‑10). Our results demonstrated that the rats had mild renal impairment consistent with a pre‑AKI stage due to the nephrotoxic effect of gentamicin. However, TOS, MDA and NO were significantly higher in the gentamicin group compared to the control group. In addition, TAC was higher in the control group. Hence, OS markers reach higher levels and may potentially be used as markers of kidney damage even in cases of mild renal function impairment. AD - Department 9‑Mother and Child, Second Clinic of Pediatrics, ‘Iuliu Haţieganu’ University of Medicine and Pharmacy, 400177 Cluj‑Napoca, Romania Department of Microbiology, ‘Iuliu Haţieganu’ University of Medicine and Pharmacy, 400012 Cluj‑Napoca, Romania Laboratory Department, ‘Prof. Dr. Octavian Fodor’ Regional Institute of Gastroenterology and Hepatology, 400162 Cluj‑Napoca, Romania Department of Pathophysiology, ‘Iuliu Haţieganu’ University of Medicine and Pharmacy, 400012 Cluj‑Napoca, Romania AU - Tomşa,Anamaria,Magdalena AU - Răchişan,Andreea,Liana AU - Pandrea,Stanca,Lucia AU - Benea,Andreea AU - Uifălean,Ana AU - Parvu,Alina,Elena AU - Junie,Lia,Monica DA - 2021/11/01 DO - 10.3892/etm.2021.10652 IS - 5 JO - Exp Ther Med KW - oxidative stress antioxidants acute kidney injury gentamicin nephrotoxicity PY - 2021 SN - 1792-0981 1792-1015 SP - 1218 ST - Accelerated lipid peroxidation in a rat model of gentamicin nephrotoxicity T2 - Experimental and Therapeutic Medicine TI - Accelerated lipid peroxidation in a rat model of gentamicin nephrotoxicity UR - https://doi.org/10.3892/etm.2021.10652 VL - 22 ER -