TY - JOUR AB - Rheumatoid arthritis (RA) is a chronic, progressive and systemic autoimmune disease mainly characterized by symmetric multijoint synovitis. Quercetin has anti‑inflammatory, anti‑oxidation and immune regulation activities, and therefore shows high medicinal value. The present study aimed to observe the effect of quercetin on fibroblast‑like synoviocytes (FLSs) in RA. Rheumatoid arthritis fibroblast‑like synoviocytes (RAFLSs) were pretreated with 50 nmol/l quercetin for 2 h and were then stimulated using TNF‑α for 24 h for subsequent experiments. RAFLSs were transfected with short interfering (si)‑X‑inactive specific transcript (XIST), microRNA (miR)‑485 mimic, miR‑485 inhibitor or si‑PSMB8 or combination. ELISA, PCR and western blotting was used to evaluate the effect of quercetin on RAFLSs treated with TNF‑α. It was revealed that quercetin inhibited the production of inflammatory cytokines and the expression of XIST in RAFLSs induced by TNF‑α. Bioinformatics analysis indicated that XIST acted as a sponge for miR‑485 and that proteasome subunit β type‑8 (PSMB8) was a direct target of miR‑485. Moreover, PSMB8 functioned as a suppressor in inflammatory cytokine production of RAFLSs induced by TNF‑α. Overall, quercetin was observed to inhibit the production of inflammatory cytokines and the expression of XIST in RAFLSs induced by TNF‑α. Moreover, XIST‑silencing could suppress the inflammatory reaction by sponging miR‑485 in cells treated with TNF‑α. Altogether, quercetin could suppress the development of RA in vitro. AD - Laboratory of New Techniques of Restoration and Reconstruction of Orthopedics and Traumatology, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, P.R. China Department of Orthopedics, Tianjin Medical University, Tianjin 300070, P.R. China Department of Orthopedics, The Third Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210001, P.R. China Department of Orthopedics, Tianjin Fifth Central Hospital, Tianjin 300450, P.R. China Laboratory of New Techniques of Restoration and Reconstruction of Orthopedics and Traumatology, The First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, P.R. China AU - Sun,Hai-Tao AU - Li,Jian-Peng AU - Qian,Wei-Qing AU - Yin,Meng-Fan AU - Yin,Hong AU - Huang,Gui-Cheng DA - 2021/11/01 DO - 10.3892/etm.2021.10695 IS - 5 JO - Exp Ther Med KW - rheumatoid arthritis quercetin X‑inactive specific transcript microRNA‑485 proteasome subunit β type‑8 PY - 2021 SN - 1792-0981 1792-1015 SP - 1260 ST - Quercetin suppresses inflammatory cytokine production in rheumatoid arthritis fibroblast‑like synoviocytes T2 - Experimental and Therapeutic Medicine TI - Quercetin suppresses inflammatory cytokine production in rheumatoid arthritis fibroblast‑like synoviocytes UR - https://doi.org/10.3892/etm.2021.10695 VL - 22 ER -