TY - JOUR AB - Human cytochrome P450 1 (CYP1) enzymes are transcriptionally induced by specific xenobiotics through a mechanism that involves the binding of aryl hydrocarbon receptors (AhR) to target xenobiotic responsive element (XRE) sequences. To examine the effect of DNA methylation on the AhR‑mediated pathway, reverse transcription‑quantitative PCR analysis was performed. β‑naphthoflavone (βNF)‑induced CYP1B1 expression was found to be potentiated by pre‑treatment of human HepG2 liver cancer cells with 5‑aza‑2'‑deoxycytidine, a DNA methyltransferase inhibitor, but not HuH7 cells. It was hypothesized that this increase is mediated by the demethylation of CpG sites within XRE2/XRE3 sequences, suggesting that methylation of these sequences inhibits gene expression by interfering with the binding of AhR to the target sequences. To test this hypothesis, a novel method combining the modified chromatin immunoprecipitation of AhR‑XRE complexes with subsequent DNA methylation analysis of the XRE regions targeted by activated AhR was applied to both liver cancer cell lines treated with βNF. XRE2/XRE3 methylation was found to be exclusively observed in the input DNA from HepG2 cells but not in the precipitated AhR‑bound DNA. Furthermore, sub‑cloning and sequencing analysis revealed that the two XRE sites were unmethylated in the samples from the AhR‑bound DNA even though the neighboring CpG sites were frequently methylated. To the best of our knowledge, the present study provides the first direct evidence that ligand‑activated AhR preferentially binds to unmethylated XRE sequences in the context of natural chromatin. In addition, this approach can also be applied to assess the effects of DNA methylation on target sequence binding by transcription factors other than AhR. AD - Division of Pharmacodynamics and Molecular Genetics, Department of Clinical Pharmaceutical Sciences, School of Pharmacy, Iwate Medical University, Shiwa, Iwate 028‑3694, Japan AU - Miura,Toshitaka AU - Onodera,Ryo AU - Terashima,Jun AU - Ozawa,Shogo AU - Habano,Wataru DA - 2021/12/01 DO - 10.3892/etm.2021.10846 IS - 6 JO - Exp Ther Med KW - aryl hydrocarbon receptor xenobiotic responsive element cytochrome P450 family 1 subfamily B member 1 β‑naphthoflavone DNA methylation PY - 2021 SN - 1792-0981 1792-1015 SP - 1410 ST - β‑naphthoflavone‑induced upregulation of CYP1B1 expression is mediated by the preferential binding of aryl hydrocarbon receptor to unmethylated xenobiotic responsive elements T2 - Experimental and Therapeutic Medicine TI - β‑naphthoflavone‑induced upregulation of CYP1B1 expression is mediated by the preferential binding of aryl hydrocarbon receptor to unmethylated xenobiotic responsive elements UR - https://doi.org/10.3892/etm.2021.10846 VL - 22 ER -