TY - JOUR AB - Long non‑coding (lnc) RNAs in circulating exosomes are a new class of promising cancer biomarkers; however, their expression in exosomes derived from gastric high‑grade intraepithelial neoplasia (GHGIN) has not been reported. In the present study, differentially expressed (DE) lncRNAs were analyzed in the peripheral blood collected from 5 patients with GHGIN and 5 healthy donors using high‑throughput sequencing. Reverse transcription‑quantitative PCR analysis was performed on 6 randomly selected DE lncRNAs to validate the reliability of the sequencing results. The potential roles of the DE lncRNAs in GHGIN were investigated using Gene Ontology (GO) and Kyoto Encyclopedia of Gene and Genome (KEGG) pathway enrichment analyses. A total of 25,145 lncRNAs were identified in all the samples and 83 DE lncRNAs were further screened, including 76 upregulated and 7 downregulated DE lncRNAs. GO and KEGG analyses predicted that the DE lncRNAs played notable roles in ‘protein/macromolecule glycosylation’, ‘regulation of protein ubiquitination’, ‘renin‑angiotensin system’ and ‘MAPK signaling pathways’. A lncRNA‑micro (mi)RNA‑mRNA interaction network was constructed and used to perform association analyses. It was found that 83 lncRNAs were abnormally expressed in GHGIN, with some potential functions associated with gastric cancer. Furthermore, the lncRNA‑miRNA‑mRNA interaction network indicated that 7 DE lncRNAs may play a notable role in the occurrence and development of GHGIN. The results of the present study showed the expression profiles of lncRNAs in human GHGIN, elucidated some of the molecular changes associated with GHGIN and improved the understanding of the molecular mechanisms underlying GHGIN and gastric cancer. AD - Department of Hepatology and Gastroenterology, The Second Part of First Hospital of Jilin University, Changchun, Jilin 130000, P.R. China Central Laboratory, The First Hospital of Jilin University, Changchun, Jilin 130000, P.R. China Intensive Care Unit, The First Hospital of Jilin University, Changchun, Jilin 130000, P.R. China Key Laboratory of Organ Regeneration and Transplantation of The Ministry of Education, Genetic Diagnosis Center, The First Hospital of Jilin University, Changchun, Jilin 130000, P.R. China AU - Hu,Feng AU - Rao,Min AU - Zhang,Manli AU - Meng,Qingqing AU - Wan,Minjie AU - Zhang,Xiuna AU - Ding,Lili AU - Jiang,Yanfang DA - 2022/01/01 DO - 10.3892/etm.2021.10923 IS - 1 JO - Exp Ther Med KW - high‑grade intraepithelial neoplasia exosomes long non‑coding RNAs high‑throughput sequencing gastric cancer PY - 2022 SN - 1792-0981 1792-1015 SP - 1 ST - Long non‑coding RNA profiles in plasma exosomes of patients with gastric high‑grade intraepithelial neoplasia T2 - Experimental and Therapeutic Medicine TI - Long non‑coding RNA profiles in plasma exosomes of patients with gastric high‑grade intraepithelial neoplasia UR - https://doi.org/10.3892/etm.2021.10923 VL - 23 ER -