TY - JOUR AB - Prostate cancer occurs in the prostatic epithelium and poses a threat to the health of middle‑aged and older males. The objective of the present study was to explore the roles of microRNA (miRNA/miR)‑130b in prostate cancer and potential molecular mechanisms in order to control the migration and invasion of prostate cancer. For this purpose, reverse transcription‑PCR was performed to evaluate the mRNA levels of DLL1, phosphoinositide‑3 kinase (PI3K), protein kinase B (Akt) and matrix metalloproteinase (MMP)9, and western blot analysis was carried out to detect the protein expression levels of DLL1, phosphorylated (p)‑PI3K, p‑Akt and MMP9. A Transwell assay was conducted to examine the invasion rate of prostate cancer cells. Furthermore, a scratch wound assay was performed to examine the migration rate of prostate cancer cells. A luciferase assay was performed to examine the interaction between miRNA and its target mRNA. The results revealed that miR‑130b had abnormal (low) expression in tumor tissues compared with that in the adjacent normal tissue. An miR‑130b mimic suppressed the expression of DLL1. The expression of p‑PI3K, p‑Akt and MMP9 in prostate cancer cells transfected with the miR‑130b mimic was decreased in comparison to the negative control and control groups. Furthermore, migration and invasion were significantly suppressed in the miR‑130b mimic group. In conclusion, a novel pathway interlinking miR‑130b and MMP9, p‑Akt and p‑PI3K, which regulates the migration and invasion of prostate cancer cells, was identified. These findings provide an intriguing biomarker and treatment strategy for patients with prostate cancer. AD - Department of Oncology, The Third Hospital of Mianyang (Sichuan Mental Health Center), Mianyang, Sichuan 621000, P.R. China Department of General Surgery, Yulin Traditional Chinese Medicine Hospital, Yulin, Shaanxi 719000, P.R. China Department of Urology Surgery, Yulin Traditional Chinese Medicine Hospital, Yulin, Shaanxi 719000, P.R. China AU - Jia,Li AU - Lei,Bin AU - Gao,Huaijun AU - Jia,Lin AU - Luo,Dan AU - Han,Jianjun AU - Jia,Bingxin DA - 2022/01/01 DO - 10.3892/etm.2021.11021 IS - 1 JO - Exp Ther Med KW - prostate cancer miR‑130b Delta‑like 1 phosphoinositide 3 kinase/protein kinase B matrix metalloproteinase 9 PY - 2022 SN - 1792-0981 1792-1015 SP - 98 ST - miR‑130b suppresses the invasion and migration of prostate cancer via inhibiting DLL1 and regulating the PI3K/Akt pathways T2 - Experimental and Therapeutic Medicine TI - miR‑130b suppresses the invasion and migration of prostate cancer via inhibiting DLL1 and regulating the PI3K/Akt pathways UR - https://doi.org/10.3892/etm.2021.11021 VL - 23 ER -