TY - JOUR
AB - The aim of the present study was to evaluate the expression levels of microRNA (miR)‑363‑3p and its underlying physiological function in oral squamous cell carcinoma (OSCC). miR‑363‑3p expression levels were measured in OSCC cell lines using reverse transcription‑quantitative PCR. The role of miR‑363‑3p in OSCC cells was examined using gain‑of‑function assays in vitro. Cell proliferation was assessed using Cell Counting Kit‑8, 5‑ethynyl‑2'‑deoxyuridine assays and flow cytometry. Cell migration and invasion were evaluated in wound‑healing and Transwell Matrigel assays. In addition, bioinformatics analysis predicted binding sites of miR‑363‑3p on sperm‑specific antigen 2 (SSFA2). Luciferase reporter and RNA pull‑down assays were conducted to test whether miR‑363‑3p interacted with SSFA2. miR‑363‑3p expression was downregulated in OSCC cell lines compared with that in the normal epithelial cell line (NHOK). Additionally, miR‑363‑3p overexpression suppressed OSCC cell proliferation, migration and invasion in vitro. SSFA2 was verified as a direct target of miR‑363‑3p, and SSFA2 overexpression partially counteracted the inhibitory effects of miR‑363‑3p on cell proliferation, migration and invasion in OSCC cell lines. Thus, miR‑363‑3p may serve as a tumor suppressor via targeting SSFA2 and may represent a potential therapeutic target for OSCC.
AD - Department of Stomatology, Yijishan Hospital, The First Affiliated Hospital of Wannan Medical College, Wuhu, Anhui 241000, P.R. China
Jiangcheng Dental Clinic, Wuhu, Anhui 241000, P.R. China
Department of Endocrinology, Wuhu Hospital of Traditional Chinese Medicine, Wuhu, Anhui 241000, P.R. China
AU - Zhu,Liangming
AU - Zhang,Lei
AU - Tang,Ying
AU - Zhang,Fang
AU - Wan,Chao
AU - Xu,Liang
AU - Guo,Ping
DA - 2021/06/01
DO - 10.3892/etm.2021.9981
IS - 6
JO - Exp Ther Med
KW - oral squamous cell carcinoma
microRNA‑363‑3p
sperm‑specific antigen 2
PY - 2021
SN - 1792-0981
1792-1015
SP - 549
ST - MicroRNA‑363‑3p inhibits tumor cell proliferation and invasion in oral squamous cell carcinoma cell lines by targeting SSFA2
T2 - Experimental and Therapeutic Medicine
TI - MicroRNA‑363‑3p inhibits tumor cell proliferation and invasion in oral squamous cell carcinoma cell lines by targeting SSFA2
UR - https://doi.org/10.3892/etm.2021.9981
VL - 21
ER -