TY - JOUR AB - Long non‑coding RNA (lncRNA) HLA complex group 22 (HCG22) is known to be involved in the occurrence and development of cancer; however, its role in oral squamous cell carcinoma (OSCC) remains unclear. Therefore, the main aim of the present study was to investigate the role and mechanisms of action of lncRNA HCG22 in OSCC cells. The expression levels of lncRNA HCG22 and microRNA (miR)‑425‑5p in OSCC cells were assessed using reverse transcription-quantitative PCR analysis. Cell proliferation was detected using Cell Counting Kit‑8 and colony formation assays. In addition, the expression levels of cell proliferation‑related proteins, p27, cyclin E and cyclin‑dependent kinase 2, were detected by western blot analysis. The cell invasive ability was detected by Transwell assay, while the cell migratory ability was detected via a wound healing assay. The expression levels of the invasion‑ and migration‑related proteins, MMP2 and MMP9, were measured by western blot analysis. The targeted association between lncRNA HCG22 and miR‑425‑5p was verified by RNA immunoprecipitation and dual‑luciferase reporter assays. The results revealed that lncRNA LCG22 was expressed at low levels, while miR‑425‑5p was highly expressed in OSCC cell lines, based on bioinformatics analysis. The overexpression of lncRNA HCG22 inhibited the proliferation, invasion and migration of OSCC cells. Moreover, lncRNA HCG22 and miR‑425‑5p were found to have a direct targeted association, and lncRNA HCG22 inhibited cell proliferation, invasion and migration by targeting miR‑425‑5p. Collectively, the findings of the present study demonstrated that lncRNA HCG22 may inhibit the proliferation, invasion and migration of OSCC cells by downregulating miR‑425‑5p expression. AD - Department of Radiology, Urumqi Stomatological Hospital, Urumqi, Xinjiang Uygur Autonomous Region 830011, P.R. China Department of General Special Requirements, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region 830011, P.R. China Department of Oncology II, Dalian Fifth People's Hospital, Dalian, Liaoning 116021, P.R. China AU - Fu,Yating AU - Liu,Ying AU - Nasiroula,Aheli AU - Wang,Qichao AU - Cao,Xinhua DA - 2022/03/01 DO - 10.3892/etm.2022.11171 IS - 3 JO - Exp Ther Med KW - oral squamous cell carcinoma long non‑coding RNA HLA complex group 22 microRNA‑425‑5p proliferation invasion migration PY - 2022 SN - 1792-0981 1792-1015 SP - 246 ST - Long non‑coding RNA HCG22 inhibits the proliferation, invasion and migration of oral squamous cell carcinoma cells by downregulating miR‑425‑5p expression T2 - Experimental and Therapeutic Medicine TI - Long non‑coding RNA HCG22 inhibits the proliferation, invasion and migration of oral squamous cell carcinoma cells by downregulating miR‑425‑5p expression UR - https://doi.org/10.3892/etm.2022.11171 VL - 23 ER -