TY - JOUR
AB - The present study aimed to investigate the anti‑inflammatory effects of quercetin and the associated mechanisms involved. ELISA, reverse transcription‑quantitative PCR and western blot analysis were performed to determine the anti‑inflammatory effects of quercetin in lipopolysaccharide (LPS)‑stimulated RAW264.7 cells. The molecular mechanisms of quercetin were investigated using network pharmacology, molecular docking technology and in vitro experiments. The results revealed that quercetin reduced the LPS‑induced production of TNF‑α, IL‑6 and IL‑1β in RAW264.7 macrophages. Protein‑protein interaction network topology analysis indicated that Akt was the target of quercetin. Kyoto Encyclopedia of Genes and Genomes analysis indicated that quercetin may regulate the PI3K/Akt signaling pathway to exert its anti‑inflammatory effects. Furthermore, the molecular docking results indicated that quercetin had a good affinity for the active sites of Akt. Western blot analysis confirmed that quercetin inhibited the phosphorylation of Akt, with an efficacy stronger than that of an Akt inhibitor. Taken together, Akt served as a target as part of the mechanism of the anti‑inflammatory effect of quercetin. This result lays a foundation for the clinical application of quercetin in the treatment of inflammatory diseases.
AD - Department of Endocrinology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200030, P.R. China
AU - Zhang,Jingwen
AU - Li,Hongyan
AU - Wang,Wei
AU - Li,Hong
DA - 2022/04/01
DO - 10.3892/etm.2022.11230
IS - 4
JO - Exp Ther Med
KW - quercetin
inflammation
network pharmacology
molecular docking
experimental validation
PY - 2022
SN - 1792-0981
1792-1015
SP - 301
ST - Assessing the anti‑inflammatory effects of quercetin using network pharmacology and in vitro experiments
T2 - Experimental and Therapeutic Medicine
TI - Assessing the anti‑inflammatory effects of quercetin using network pharmacology and in vitro experiments
UR - https://doi.org/10.3892/etm.2022.11230
VL - 23
ER -