TY - JOUR AB - The present study aimed to investigate the anti‑inflammatory effects of quercetin and the associated mechanisms involved. ELISA, reverse transcription‑quantitative PCR and western blot analysis were performed to determine the anti‑inflammatory effects of quercetin in lipopolysaccharide (LPS)‑stimulated RAW264.7 cells. The molecular mechanisms of quercetin were investigated using network pharmacology, molecular docking technology and in vitro experiments. The results revealed that quercetin reduced the LPS‑induced production of TNF‑α, IL‑6 and IL‑1β in RAW264.7 macrophages. Protein‑protein interaction network topology analysis indicated that Akt was the target of quercetin. Kyoto Encyclopedia of Genes and Genomes analysis indicated that quercetin may regulate the PI3K/Akt signaling pathway to exert its anti‑inflammatory effects. Furthermore, the molecular docking results indicated that quercetin had a good affinity for the active sites of Akt. Western blot analysis confirmed that quercetin inhibited the phosphorylation of Akt, with an efficacy stronger than that of an Akt inhibitor. Taken together, Akt served as a target as part of the mechanism of the anti‑inflammatory effect of quercetin. This result lays a foundation for the clinical application of quercetin in the treatment of inflammatory diseases. AD - Department of Endocrinology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200030, P.R. China AU - Zhang,Jingwen AU - Li,Hongyan AU - Wang,Wei AU - Li,Hong DA - 2022/04/01 DO - 10.3892/etm.2022.11230 IS - 4 JO - Exp Ther Med KW - quercetin inflammation network pharmacology molecular docking experimental validation PY - 2022 SN - 1792-0981 1792-1015 SP - 301 ST - Assessing the anti‑inflammatory effects of quercetin using network pharmacology and in vitro experiments T2 - Experimental and Therapeutic Medicine TI - Assessing the anti‑inflammatory effects of quercetin using network pharmacology and in vitro experiments UR - https://doi.org/10.3892/etm.2022.11230 VL - 23 ER -