TY - JOUR AB - The dysfunction of renal mesangial cells (MCs) is a hallmark of diabetic kidney disease (DKD), which triggers glomerulosclerosis leading to end‑stage renal disease. Procyanidin B2 (PB2), the main component of proanthocyanidin, is well known for its antioxidant and anti‑inflammatory effects; however, it remains unclear as to whether it has protective effects on DKD. The present study investigated the protective effect of PB2 against hyperglycemia‑induced renal MC dysfunction in mouse SV40‑Mes13 (Mes13) cells. The Mes13 cells were treated with or without PB2 under HG conditions. Cell proliferation was assessed using an MTT assay and oxidative stress was assessed by examining intracellular ROS generation and H2O2 production. The changes in extracellular matrix accumulation‑ and cellular inflammation‑related proteins were measured by western blot analysis, ELISA and immunofluorescence analysis. The results showed that PB2 treatment markedly attenuated hyperglycemia‑induced cell proliferation, oxidative stress, extracellular matrix accumulation and cellular inflammation in Mes13 cells, which was accompanied by an inactivation of redoxosomes, TGF‑β1/SMAD and IL‑1β/TNF‑α/NF‑κB signaling pathways. The present study also demonstrated that hyperglycemia upregulated and activated caveolin‑1 (CAV‑1), whereas PB2 treatment potently reversed this effect. In accordance, CAV‑1 overexpression abolished the protective effects of PB2 against hyperglycemia in Mes13 cells, indicating that the cytoprotective effect of PB2 was CAV‑1‑dependent. These findings form the basis of the potential clinical applications of PB2 in the treatment of DKD. AD - Department of Nephrology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China NHC Key Laboratory of Nuclear Medicine, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi, Jiangsu 214063, P.R. China Department of Nephrology, People's Hospital of Suzhou New District, Suzhou, Jiangsu 215129, P.R. China Department of Nephrology, The Affiliated Wuxi No.2 People's Hospital of Nanjing Medical University, Wuxi, Jiangsu 214002, P.R. China Sydney Pharmacy School, The University of Sydney, Sydney, New South Wales A-2006, Australia AU - Yin,Jun AU - Wang,Ke AU - Zhu,Xue AU - Lu,Guoyuan AU - Jin,Donghua AU - Qiu,Junsi AU - Zhou,Fanfan DA - 2022/08/01 DO - 10.3892/etm.2022.11423 IS - 2 JO - Exp Ther Med KW - diabetic kidney disease renal mesangial cells procyanidin B2 caveolin‑1 PY - 2022 SN - 1792-0981 1792-1015 SP - 496 ST - Procyanidin B2 suppresses hyperglycemia‑induced renal mesangial cell dysfunction by modulating CAV‑1‑dependent signaling T2 - Experimental and Therapeutic Medicine TI - Procyanidin B2 suppresses hyperglycemia‑induced renal mesangial cell dysfunction by modulating CAV‑1‑dependent signaling UR - https://doi.org/10.3892/etm.2022.11423 VL - 24 ER -